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| Status |
Public on Dec 13, 2021 |
| Title |
m_WT1andWT2 [SC121892] |
| Sample type |
SRA |
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| Source name |
heart
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
sample type: heart
genotype/variation: WT
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Cell suspension was centrifuge (100 x g, 2min) three times to separate the cardiomyocytes and non-cardiomyocytes
One ng of purified cDNA was used to generate a sequencing library with the Nextera XT DNA sample preparation Kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Raw reads were filtered by cutadpt v1.8.1,-m 20 --trim-n --max-n 0.7 -q 20
Contaminants were filtered by fastq_screen (v0.5.1. 4)
Clean reads were mapped to UCSC mm10 genome via STAR v2.5.2b
Reads were assigned to Ensembl genes by featureCounts (subread-1.4.6-p1 command line)
Genome_build: mm10
Supplementary_files_format_and_content: csv,gene expression matrix for single cell RNASeq
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| Submission date |
Aug 11, 2021 |
| Last update date |
Dec 13, 2021 |
| Contact name |
Li Wang |
| Organization name |
NIH
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| Street address |
111 T.W. Alexander Dr
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| City |
Durham |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE181935 |
Leukotriene B4 receptor 1 antagonist in combination with kinase MST1/2 inhibitor has potential for treatment of myocardial infarction |
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| Relations |
| BioSample |
SAMN20717371 |
| SRA |
SRX11721982 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5514596_121892_S1.R1.fastq_merged.genematrix.csv.gz |
7.6 Mb |
(ftp)(http) |
CSV |
| GSM5514596_121892_S1.R1.fastq_merged.report.csv.gz |
85.7 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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