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Sample GSM5469353 Query DataSets for GSM5469353
Status Public on Jul 24, 2021
Title autoantigen_V2_Patient SU10 V4
Sample type protein
 
Source name Hospitalized COVID-19
Organism Homo sapiens
Characteristics tissue: serum
condition: Hospitalized COVID-19
Extracted molecule protein
Extraction protocol Blood was collected from patients and healthy controls from 3 different centers. The blood was then processed into serum which was then run on our arrays.
Label R-PE conjugated goat anti-human IgG secondary antibody
Label protocol A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from serum samples.
 
Hybridization protocol A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from serum samples.
Scan protocol A Luminex FlexMAP 3D instrument was used to measure fluorescence (in MFI) from each barcoded bead.
Description Antibodies in patient serum samples bind to conjugated beads in protein array. The fluorescence measured as MFI from the secondary antibody was used to determine serum antibody reactivity.
Data processing Sample replicate MFI values were averaged for each antigen. The average barebead MFI was then subtracted from the average MFI of each sample and antigen.
 
Submission date Jul 23, 2021
Last update date Jul 24, 2021
Contact name Allan Feng
E-mail(s) afeng1@stanford.edu
Phone 2055678956
Organization name Stanford School of Medicine
Department Imm/Rheum
Lab Utz
Street address 269 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL30431
Series (2)
GSE180738 New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19 [Autoantigen V2]
GSE180743 New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19

Data table header descriptions
ID_REF
VALUE Each numerical value represents the mean fluorescent intensity (MFI) of each sample and bead after data processing.

Data table
ID_REF VALUE
6 3435.25
7 0
8 6888.75
9 0
10 2493.5
11 2116.25
12 47.25
13 768.5
14 3993.75
15 9142
16 4153.5
17 4965.25
18 387.25
19 0
20 0
21 0
22 0
23 0
24 0
25 4164

Total number of rows: 53

Table truncated, full table size <1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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