|
Status |
Public on Jul 24, 2021 |
Title |
autoantigen_V1_Healthy Control HC32 |
Sample type |
protein |
|
|
Source name |
Healthy Control
|
Organism |
Homo sapiens |
Characteristics |
tissue: serum condition: Healthy Control
|
Extracted molecule |
protein |
Extraction protocol |
Blood was collected from patients and healthy controls from 3 different centers. The blood was then processed into serum which was then run on our arrays.
|
Label |
R-PE conjugated goat anti-human IgG secondary antibody
|
Label protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from serum samples.
|
|
|
Hybridization protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from serum samples.
|
Scan protocol |
A Luminex FlexMAP 3D instrument was used to measure fluorescence (in MFI) from each barcoded bead.
|
Description |
Antibodies in patient serum samples bind to conjugated beads in protein array. The fluorescence measured as MFI from the secondary antibody was used to determine serum antibody reactivity.
|
Data processing |
Sample replicate MFI values were averaged for each antigen. The average barebead MFI was then subtracted from the average MFI of each sample and antigen.
|
|
|
Submission date |
Jul 23, 2021 |
Last update date |
Jul 24, 2021 |
Contact name |
Allan Feng |
E-mail(s) |
afeng1@stanford.edu
|
Phone |
2055678956
|
Organization name |
Stanford School of Medicine
|
Department |
Imm/Rheum
|
Lab |
Utz
|
Street address |
269 Campus Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL30430 |
Series (2) |
GSE180737 |
New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19 [Autoantigen V1] |
GSE180743 |
New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19 |
|