|
Status |
Public on Nov 25, 2011 |
Title |
wtns_3 |
Sample type |
RNA |
|
|
Source name |
wtns_3
|
Organism |
Homo sapiens |
Characteristics |
genotype: wild type harvest date: 20-10-09 treatment: no treatment cell line: HEK-293
|
Treatment protocol |
no treatment
|
Growth protocol |
cell culture/primary cell - MEM (PAA Laboratory GmbH) + Fetal Bovin Serum 10% + Penicilin and Streptomycin 1% 37°C; 5% C02
|
Extracted molecule |
total RNA |
Extraction protocol |
wtns_3:92.3ug.
|
Label |
Biotin
|
Label protocol |
labelling Biotin direct, amplification=yes, cRNA 20 ug.
|
|
|
Hybridization protocol |
labelled extract quantity: 15ug
|
Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
Description |
- G protein-coupled receptors (GPCR) are centrally involved in most physiological processes and are a major drug targets. They transduce extracellular signals inside the cells through at least two different mechanisms: i) the classical coupling to heterotrimeric G proteins and ii) a newly discovered -arrestin-dependent pathway. The fundamental issue of the respective impacts that these two transduction mechanisms exert on gene regulation has not been clearly addressed to date. To tackle this question, we have developed two mutants of the follicle stimulating hormone (FSH) receptors which do not couple to G proteins upon FSH activation but continue to recruit -arrestins and signal through them.
|
Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
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|
|
Submission date |
May 25, 2010 |
Last update date |
Apr 30, 2013 |
Contact name |
sandrine balzergue |
Organization name |
INRA
|
Lab |
IRHS
|
Street address |
Rue G. Morel
|
City |
Beaucouze |
ZIP/Postal code |
49000 |
Country |
France |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE21982 |
Impact of G protein versus beta-arrestin-dependent signal transduction at the FSH receptor (affy_fsh_human) |
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