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Sample GSM546595 Query DataSets for GSM546595
Status Public on Nov 25, 2011
Title wtns_3
Sample type RNA
 
Source name wtns_3
Organism Homo sapiens
Characteristics genotype: wild type
harvest date: 20-10-09
treatment: no treatment
cell line: HEK-293
Treatment protocol no treatment
Growth protocol cell culture/primary cell - MEM (PAA Laboratory GmbH) + Fetal Bovin Serum 10% + Penicilin and Streptomycin 1% 37°C; 5% C02
Extracted molecule total RNA
Extraction protocol wtns_3:92.3ug.
Label Biotin
Label protocol labelling Biotin direct, amplification=yes, cRNA 20 ug.
 
Hybridization protocol labelled extract quantity: 15ug
Scan protocol GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
Description - G protein-coupled receptors (GPCR) are centrally involved in most physiological processes and are a major drug targets. They transduce extracellular signals inside the cells through at least two different mechanisms: i) the classical coupling to heterotrimeric G proteins and ii) a newly discovered -arrestin-dependent pathway. The fundamental issue of the respective impacts that these two transduction mechanisms exert on gene regulation has not been clearly addressed to date. To tackle this question, we have developed two mutants of the follicle stimulating hormone (FSH) receptors which do not couple to G proteins upon FSH activation but continue to recruit -arrestins and signal through them.
Data processing The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
 
Submission date May 25, 2010
Last update date Apr 30, 2013
Contact name sandrine balzergue
Organization name INRA
Lab IRHS
Street address Rue G. Morel
City Beaucouze
ZIP/Postal code 49000
Country France
 
Platform ID GPL571
Series (1)
GSE21982 Impact of G protein versus beta-arrestin-dependent signal transduction at the FSH receptor (affy_fsh_human)

Data table header descriptions
ID_REF ID number
VALUE Normalized data by GCRMA

Data table
ID_REF VALUE
1007_s_at 7.927160566
1053_at 10.24062447
117_at 2.608124151
121_at 2.395330047
1255_g_at 2.207905352
1294_at 2.274324085
1316_at 2.408700253
1320_at 2.392411054
1405_i_at 2.498136428
1431_at 2.331900613
1438_at 2.088763557
1487_at 6.314378185
1494_f_at 2.073417402
1598_g_at 4.255757665
160020_at 2.215607753
1729_at 3.39874874
1773_at 2.059484051
177_at 2.425740885
179_at 1.990602017
1861_at 7.106062445

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM546595_wtns_3.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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