|
Status |
Public on Sep 30, 2021 |
Title |
S1067__00019 |
Sample type |
RNA |
|
|
Source name |
Breast cancer pre-treatment biopsy
|
Organism |
Homo sapiens |
Characteristics |
tissue: Breast cancer
|
Treatment protocol |
All baseline samples were taken prior to any anti-cancer treatment
|
Extracted molecule |
total RNA |
Extraction protocol |
Extraction of total RNA was carried out in accordance with standard SOPs
|
Label |
Biotin
|
Label protocol |
3.5 µg biotinylated RNA was prepared using the NuGEN Encore Biotin Module
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|
|
Hybridization protocol |
Following fragmentation, RNA was hybridised onto Affymetrix GeneChip arrays. GeneChips were washed and stained in the Affymetrix GeneChips fluidics stating 450
|
Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000
|
Description |
Baseline diagnostic core biopsy Gene expression data from breast cancer pre-treatment Baseline tumour core biopsies were taken from breast cancers under ultrasound guidance
|
Data processing |
Microarray data was processed using the Robust Multi-array average method.
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|
|
Submission date |
Jul 17, 2021 |
Last update date |
Sep 30, 2021 |
Contact name |
Stuart McIntosh |
E-mail(s) |
s.mcintosh@qub.ac.uk
|
Organization name |
Queen's University Belfast
|
Lab |
Stuart McIntosh
|
Street address |
97 Lisburn Road
|
City |
Belfast |
ZIP/Postal code |
BT9 7AE |
Country |
United Kingdom |
|
|
Platform ID |
GPL30404 |
Series (1) |
GSE180295 |
Array data from neoDDRD clinical trial |
|