|
| Status |
Public on Sep 30, 2021 |
| Title |
S1067__00015 |
| Sample type |
RNA |
| |
|
| Source name |
Breast cancer pre-treatment biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Breast cancer
|
| Treatment protocol |
All baseline samples were taken prior to any anti-cancer treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was carried out in accordance with standard SOPs
|
| Label |
Biotin
|
| Label protocol |
3.5 µg biotinylated RNA was prepared using the NuGEN Encore Biotin Module
|
| |
|
| Hybridization protocol |
Following fragmentation, RNA was hybridised onto Affymetrix GeneChip arrays. GeneChips were washed and stained in the Affymetrix GeneChips fluidics stating 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000
|
| Description |
Baseline diagnostic core biopsy
Gene expression data from breast cancer pre-treatment
Baseline tumour core biopsies were taken from breast cancers under ultrasound guidance
|
| Data processing |
Microarray data was processed using the Robust Multi-array average method.
|
| |
|
| Submission date |
Jul 17, 2021 |
| Last update date |
Sep 30, 2021 |
| Contact name |
Stuart McIntosh |
| E-mail(s) |
s.mcintosh@qub.ac.uk
|
| Organization name |
Queen's University Belfast
|
| Lab |
Stuart McIntosh
|
| Street address |
97 Lisburn Road
|
| City |
Belfast |
| ZIP/Postal code |
BT9 7AE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL30404 |
| Series (1) |
| GSE180295 |
Array data from neoDDRD clinical trial |
|
