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Sample GSM5448541 Query DataSets for GSM5448541
Status Public on Sep 01, 2021
Title D28_preg_1
Sample type SRA
Source name pregnant mare
Organism Equus caballus
Characteristics tissue: endometrium
Sex: female
days after ovulation: 28
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNEasy Mini kit. TruSeq Stranded mRNA Library Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Sequenced reads were trimmed for adaptor sequence and low quality ends and reads shorter than 50 bp were removed
Reads were then mapped to the equine EquCab3 genome allowing two mismatches per read
Total counts and transcripts per million (TPM) were generated for all transcripts
Genome_build: EquCab3
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
Submission date Jul 13, 2021
Last update date Sep 01, 2021
Contact name Claudia Klein
Phone 050348715135
Organization name Friedrich-Loeffler-Institut, Institute of Farm Animal Genetics
Street address Höltystr. 10
City Neustadt
ZIP/Postal code 31535
Country Germany
Platform ID GPL21401
Series (1)
GSE180022 Transcriptional profiling of equine endometrium before, during and after capsule disintegration during normal pregnancy and after oxytocin-induced luteostasis in non-pregnant mares
BioSample SAMN20199235
SRA SRX11431737

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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