|
| Status |
Public on Jun 30, 2024 |
| Title |
kidney samples of controls [163] |
| Sample type |
RNA |
| |
|
| Source name |
kidney samples of controls
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
subject status: control
tissue: kidney
|
| Treatment protocol |
three samples of DKDpatients and three samples of controls were performed the mircoassay analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
On average, 50 7 um-thick cryostat sections were prepared for the extraction of total RNA from tumor cell-rich areas, which were identified by a surgical pathologist (YY) using every 10th section stained by May-Giemsa. Careful attention was paid to the mi
|
| Label |
33P
|
| Label protocol |
Five mg of total RNA was reverse-transcribed using oligo-dT primers (Invitrogen) and SuperScript II reverse transcriptase (Invitrogen) in the presence of 100 mCi of [33P]dCTP (Amersham Bioscience, Piscataway, NJ) according to the instructions for the Gene
|
| |
|
| Hybridization protocol |
The GeneFilters were prehybridized for 2 h at 51C with 0.5 mg/ml of poly-dA (Invitrogen) and 0.5 mg/ml Cot-1 DNA (Invitrogen) in 10 ml of AlkPhos DIRECT hybridization buffer (Amersham Bioscience) and then hybridized for 17 h at 51C with the denatured radi
|
| Scan protocol |
The arrays were then exposed for 2 hours to an Imaging Plate and scanned at 25-mm resolution with a BAS5000 phosphoimager (Fuji Photo Film), images of the hybridized arrays were processed with L Process (Fuji Photo Film) and signal intensities quantified
|
| Description |
Total genomic DNA was spotted on 192 spots for positive control. Nothing was spotted on 40 spot with the aim of negative control. Therefore, a total of 232 spot data were already eliminated from the whole 5584 spot data on the GF201 array.
|
| Data processing |
The raw data were re-scaled to account for the differences in individual hybridization intensities. We employed a rank-invariant scaling method to select genes (Tseng et al., 2001), which were then used for fitting of a non-linear normalization curve. The normalized_data.txt contains normalized signal intensity.
|
| |
|
| Submission date |
Jun 30, 2021 |
| Last update date |
Jun 30, 2024 |
| Contact name |
Guo Jia |
| Organization name |
The First Affiliated Hospital of Zhengzhou University
|
| Street address |
1 Jianshe Dong Lu, Erqi District,
|
| City |
zhengzhou |
| ZIP/Postal code |
450052 |
| Country |
China |
| |
|
| Platform ID |
GPL30330 |
| Series (1) |
| GSE179171 |
miRNA expression of kidney samples of DKD patients and controls |
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