|
| Status |
Public on Mar 02, 2011 |
| Title |
Gata4 kd rep3 [genex] |
| Sample type |
RNA |
| |
|
| Source name |
HL-1 cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell line: HL-1 cardiac muscle cell line
protocol: Gata4 knockdown
|
| Treatment protocol |
HL-1 cells were infected with adenovirus expressing control siRNA or Gata4 and Mef2a-Gata4 and siRNA for 72 hrs before RNA extraction and Dnase I treatment followed by Mouse Gene expression ST 1.0 arrays.
|
| Growth protocol |
HL-1 cells were cultured as described by Claycomb, and split 1:3 every 3-4 days until full confluence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol then Rneasy
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol was used.
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol was used.
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner 3000.
|
| Description |
HL-1 cells infected with adenovirus expressing Gata4 siRNA.
|
| Data processing |
The data were analyzed with Affymetrix Power Tools version 1.10 using Affymetrix default analysis settings of PLIER algorithm and quantile normalization method.
|
| |
|
| Submission date |
Apr 27, 2010 |
| Last update date |
Mar 02, 2011 |
| Contact name |
Sek Won Kong |
| E-mail(s) |
swkong@enders.tch.harvard.edu
|
| Phone |
617-919-2689
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Lab |
EN137
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE21529 |
Cardiac transcription factors in HL-1 cells: gene expression and genome binding profiling |
|
