|
Status |
Public on Jan 21, 2022 |
Title |
Control_GM_BR3_RNAseq |
Sample type |
SRA |
|
|
Source name |
Murine myoblasts C2C12
|
Organism |
Mus musculus |
Characteristics |
cell line: C2C12 genotype/variation: Wild-type tissue: Myoblasts
|
Growth protocol |
C2C12 PAX7 NEG cells were maintained in HyClone Dulbecco’s High Glucose Modified Eagles Medium (GE #SH30081.01) with 20% FBS. For differentiation to myotubes serum was exchanged to 2%HS.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Cells were grown to ~80% confluency for GM or differentiated for 3 days for DM3 and washed briefly twice with ice-cold PBS. Total RNA was extracted by Trizol reagent (Invitrogen #15596018) and purified with column-based Direct-zol RNA Miniprep kit (Zymo Research #R2051). RNA-seq was performed by Hudson Alpha on poly(A) enriched RNA using the Illumina HiSeq 2500 instrument.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
RNAseq_fpkm_all_samples.txt
|
Data processing |
STAR HTSeq DESeq2 Genome_build: mm10 Supplementary_files_format_and_content: .txt: table with fpkm for all samples
|
|
|
Submission date |
May 10, 2021 |
Last update date |
Jan 21, 2022 |
Contact name |
Roza Kamila Przanowska |
Organization name |
University of Virginia
|
Department |
Biomedical Engineering
|
Street address |
1340 JPA
|
City |
Charlottesville |
State/province |
Virginia |
ZIP/Postal code |
22908 |
Country |
USA |
|
|
Platform ID |
GPL17021 |
Series (2) |
GSE174203 |
Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors [RNA-seq] |
GSE174218 |
Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors |
|
Relations |
BioSample |
SAMN19102676 |
SRA |
SRX10833276 |