GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM527050 Query DataSets for GSM527050
Status Public on Oct 22, 2021
Title Preteated PBD150 inhibitor before the addition LPS (4μg/ml) treatment biol rep 1 tech rep 2
Sample type RNA
Source name U937 cell line (human histiocytic lymphoma cell)
Organism Homo sapiens
Characteristics cell line: U937
treatment: Preteated PBD150 inhibitor before the addition LPS (4μg/ml)
Treatment protocol Human macrophage cells were untreatment, LPS (4μg/ml) only, preteated PBD150 inhibitor (10μM) for 2 hrs before the addition LPS (4μg/ml) treatment, the incubation time of the culture was 48 hours.
Growth protocol The U937 cells were maintained at 2-3 × 106 cells/ml in a RPMI 1640 medium supplemented with 10% FBS, the incubation time of the culture was 48 hours.
Extracted molecule total RNA
Extraction protocol RNA extraction by column based extraction kit (RiboPure-Blood, Ambion). RNA purity was checked by optical density of NanoDrop ND-1000 and agarose electrophoresis. RNA integrity was measured by Agilent RNA 6000 Nano Assay (RIN>8).
Label Cy5
Label protocol 2.5 µg of total RNA was reverse-transcribed and amplified using MessageAmpTM aRNA Amplification Kit (Ambion). Indirect labeling the aa-UTP whereon by NHS-CyDye (Cy5, Amershan).
Hybridization protocol HOA v4.3 arrays were pre-heat at 60℃ for 10 mins, rehydrated by 100% ethanol following with deionized water. The slides were pre-hybridized with 5x SSPE, 0.1% SDS and 1% BSA at 42℃ for 2 hour. After the pre-hybridization, 5 µg Cy5-labeled aRNA was hybridize on HOA in the presentation of the Phalanx OneArray hybridization buffer.
Scan protocol The arrays were scanned by Axon GenePix 4000B scanner (635nm power 100 PMT 600~630 ; 532nm power 10, PMT 460) and quantify the fluoresence intensity.
Description Phalanx Biotech Group's Human OneArray contains 32,048 features, 30968 detection probes and 1099 control probes, spotted onto glass slides using a proprietary non-contact printing method.
Data processing The raw data were adjust by Rosetta Resolver® error model calculation. The statistic values were calculated after the replicated probes queezing and median scaling normalization.
Submission date Mar 26, 2010
Last update date Oct 22, 2021
Contact name Chen Yi Ling
Organization name Academia Sinica
Department Institute of Biological Chemistry
Lab 501
Street address 128 Academia Road, Section 2
City Taipei
ZIP/Postal code 11529
Country Taiwan
Platform ID GPL6254
Series (1)
GSE21082 Glutaminyl cyclase (QC) activity affects gene expression in macrophages during inflammation

Data table header descriptions
VALUE Rosetta software computed normalized signal intensity

Data table
PH_hs_0000002 1224.67871
PH_hs_0000003 7.21106243
PH_hs_0000004 100.954872
PH_hs_0000005 -2.40368748
PH_hs_0000006 33857.1406
PH_hs_0000007 1871.27075
PH_hs_0000008 331.708862
PH_hs_0000009 912.199402
PH_hs_0000010 17632.25
PH_hs_0000011 313.681213
PH_hs_0000012 8987.3877
PH_hs_0000013 759.565247
PH_hs_0000014 618.949524
PH_hs_0000015 170.661804
PH_hs_0000016 86.5327454
PH_hs_0000017 18.0276566
PH_hs_0000018 106.964096
PH_hs_0000019 10.8165932
PH_hs_0000020 7612.47803
PH_hs_0000021 181.478409

Total number of rows: 30968

Table truncated, full table size 752 Kbytes.

Supplementary file Size Download File type/resource
GSM527050_H50-0607008423.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap