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Sample GSM527049 Query DataSets for GSM527049
Status Public on Oct 22, 2021
Title Preteated PBD150 inhibitor before the addition LPS (4μg/ml) treatment biol rep 1 tech rep 1
Sample type RNA
Source name U937 cell line (human histiocytic lymphoma cell)
Organism Homo sapiens
Characteristics cell line: U937
treatment: Preteated PBD150 inhibitor before the addition LPS (4μg/ml)
Treatment protocol Human macrophage cells were untreatment, LPS (4μg/ml) only, preteated PBD150 inhibitor (10μM) for 2 hrs before the addition LPS (4μg/ml) treatment, the incubation time of the culture was 48 hours.
Growth protocol The U937 cells were maintained at 2-3 × 106 cells/ml in a RPMI 1640 medium supplemented with 10% FBS, the incubation time of the culture was 48 hours.
Extracted molecule total RNA
Extraction protocol RNA extraction by column based extraction kit (RiboPure-Blood, Ambion). RNA purity was checked by optical density of NanoDrop ND-1000 and agarose electrophoresis. RNA integrity was measured by Agilent RNA 6000 Nano Assay (RIN>8).
Label Cy5
Label protocol 2.5 µg of total RNA was reverse-transcribed and amplified using MessageAmpTM aRNA Amplification Kit (Ambion). Indirect labeling the aa-UTP whereon by NHS-CyDye (Cy5, Amershan).
Hybridization protocol HOA v4.3 arrays were pre-heat at 60℃ for 10 mins, rehydrated by 100% ethanol following with deionized water. The slides were pre-hybridized with 5x SSPE, 0.1% SDS and 1% BSA at 42℃ for 2 hour. After the pre-hybridization, 5 µg Cy5-labeled aRNA was hybridize on HOA in the presentation of the Phalanx OneArray hybridization buffer.
Scan protocol The arrays were scanned by Axon GenePix 4000B scanner (635nm power 100 PMT 600~630 ; 532nm power 10, PMT 460) and quantify the fluoresence intensity.
Description Phalanx Biotech Group's Human OneArray contains 32,048 features, 30968 detection probes and 1098 control probes, spotted onto glass slides using a proprietary non-contact printing method.
Data processing The raw data were adjust by Rosetta Resolver® error model calculation. The statistic values were calculated after the replicated probes queezing and median scaling normalization.
Submission date Mar 26, 2010
Last update date Oct 22, 2021
Contact name Chen Yi Ling
Organization name Academia Sinica
Department Institute of Biological Chemistry
Lab 501
Street address 128 Academia Road, Section 2
City Taipei
ZIP/Postal code 11529
Country Taiwan
Platform ID GPL6254
Series (1)
GSE21082 Glutaminyl cyclase (QC) activity affects gene expression in macrophages during inflammation

Data table header descriptions
VALUE Rosetta software computed normalized signal intensity

Data table
PH_hs_0000002 930.513184
PH_hs_0000003 19.1546593
PH_hs_0000004 143.155869
PH_hs_0000005 16.1302395
PH_hs_0000006 30148.4258
PH_hs_0000007 1954.78345
PH_hs_0000008 304.458282
PH_hs_0000009 531.289734
PH_hs_0000010 13790.3467
PH_hs_0000011 332.686188
PH_hs_0000012 7040.84961
PH_hs_0000013 784.332886
PH_hs_0000014 712.754944
PH_hs_0000015 165.334961
PH_hs_0000016 95.7733002
PH_hs_0000017 32.260479
PH_hs_0000018 28.2279186
PH_hs_0000019 -1.00813997
PH_hs_0000020 5295.75928
PH_hs_0000021 183.481476

Total number of rows: 30968

Table truncated, full table size 752 Kbytes.

Supplementary file Size Download File type/resource
GSM527049_H49-0607008422.gpr.gz 2.7 Mb (ftp)(http) GPR
Processed data included within Sample table

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