|
| Status |
Public on Apr 24, 2021 |
| Title |
Bisulfite-seq_Col-0 rep1 |
| Sample type |
SRA |
| |
|
| Source name |
12 days old seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: wild type
tissue: seedlings
developmental stage: 12 days old
|
| Treatment protocol |
no treatment
|
| Growth protocol |
Seeds were stratified 48h at 4°C, and grown on soil in a plant growth chamber under long day light conditions (16h light /8h dark) at (22°C day /18°C night).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
12 days old seedling were quick freeze in liquid nitrogen, then grind with mortar. DNA were harvested.
DNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
RANDOM |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
BS-Seeker2 v2.1.0
Genome_build: Athaliana_167_TAIR9
Supplementary_files_format_and_content: BigWig format, contains methylation values over genome coordinates, can be visualized in Integrated Genome Browser, https://www.bioviz.org/
|
| |
|
| Submission date |
Apr 23, 2021 |
| Last update date |
Apr 25, 2021 |
| Contact name |
Bin Sun |
| E-mail(s) |
SunBin0909@hotmail.com
|
| Organization name |
Copenhagen Plant Science Centre – University of Copenhagen
|
| Department |
Department of Plant and Environmental Sciences
|
| Lab |
Stephan Wenkel's Lab
|
| Street address |
Thorvaldsensvej 40
|
| City |
Frederiksberg C |
| ZIP/Postal code |
1871 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL13222 |
| Series (2) |
| GSE173190 |
Bisulfte sequnencing of Wild Type (Col-0), and miP1aOX in Arabidopsis thaliana |
| GSE173192 |
Bisulfte sequnencing and DNA-seq of miP1aOX in Arabidopsis thaliana |
|
| Relations |
| BioSample |
SAMN18844843 |
| SRA |
SRX10665038 |
