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Sample GSM5233278 Query DataSets for GSM5233278
Status Public on Jun 28, 2021
Title fetal Leydig cells S3
Sample type SRA
 
Source name testis
Organism Mus musculus
Characteristics genotype: Nr5a1 Tg
Stage: E18.5
replicate: 3
Sex: Male
Extracted molecule total RNA
Extraction protocol Tissues were enzymatically dissociated with Trysine-EDTA and then filtered (70µm). The single cell solution is then sorted by fluorescent-active cell sorting to obtain a solution of alive and single Nr5a1-GFP+ cells. RNA was extracted from these cells with RNeasy Mini kit (Qiagen).
Sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold (Ribo-Zero), with 3 replicates by condition.
Sequencing with Illumina HiSeq 2500 (50 bp, paired-end)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description Sex: XY
bulk RNA sequencing Leydig cells
FLcs_vs_ALCs_DESEq2_results.csv
Data processing Reads were demultiplexed with Casava (v1.8.2)
Mapped with GemTools (v1.7.1)
Read counts and RPKM gene expression quantifications were calculated with an in-house pipeline based on Gencode annotation GRCm38 (v4)
Genome_build: GRCm38 (mm10)
Supplementary_files_format_and_content: counts and rpkm matrices
Supplementary_files_format_and_content: rMATS (splicing events) output
 
Submission date Apr 08, 2021
Last update date Jun 29, 2021
Contact name Serge Nef
E-mail(s) serge.nef@unige.ch
Organization name University of Geneva
Department Genetic Medicine and Development
Street address Rue Michel-Servet 1
City Genève
ZIP/Postal code 1206
Country Switzerland
 
Platform ID GPL17021
Series (1)
GSE171746 Bulk RNA-seq of fetal and adult murine Leydig cells
Relations
BioSample SAMN18677466
SRA SRX10559532

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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