|
Status |
Public on Jun 28, 2021 |
Title |
fetal Leydig cells S3 |
Sample type |
SRA |
|
|
Source name |
testis
|
Organism |
Mus musculus |
Characteristics |
genotype: Nr5a1 Tg Stage: E18.5 replicate: 3 Sex: Male
|
Extracted molecule |
total RNA |
Extraction protocol |
Tissues were enzymatically dissociated with Trysine-EDTA and then filtered (70µm). The single cell solution is then sorted by fluorescent-active cell sorting to obtain a solution of alive and single Nr5a1-GFP+ cells. RNA was extracted from these cells with RNeasy Mini kit (Qiagen). Sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold (Ribo-Zero), with 3 replicates by condition. Sequencing with Illumina HiSeq 2500 (50 bp, paired-end)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
Sex: XY bulk RNA sequencing Leydig cells FLcs_vs_ALCs_DESEq2_results.csv
|
Data processing |
Reads were demultiplexed with Casava (v1.8.2) Mapped with GemTools (v1.7.1) Read counts and RPKM gene expression quantifications were calculated with an in-house pipeline based on Gencode annotation GRCm38 (v4) Genome_build: GRCm38 (mm10) Supplementary_files_format_and_content: counts and rpkm matrices Supplementary_files_format_and_content: rMATS (splicing events) output
|
|
|
Submission date |
Apr 08, 2021 |
Last update date |
Jun 29, 2021 |
Contact name |
Serge Nef |
E-mail(s) |
serge.nef@unige.ch
|
Organization name |
University of Geneva
|
Department |
Genetic Medicine and Development
|
Street address |
Rue Michel-Servet 1
|
City |
Genève |
ZIP/Postal code |
1206 |
Country |
Switzerland |
|
|
Platform ID |
GPL17021 |
Series (1) |
GSE171746 |
Bulk RNA-seq of fetal and adult murine Leydig cells |
|
Relations |
BioSample |
SAMN18677466 |
SRA |
SRX10559532 |