|
| Status |
Public on Jun 28, 2021 |
| Title |
fetal Leydig cells S2 |
| Sample type |
SRA |
| |
|
| Source name |
testis
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Nr5a1 Tg
Stage: E18.5
replicate: 2
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were enzymatically dissociated with Trysine-EDTA and then filtered (70µm). The single cell solution is then sorted by fluorescent-active cell sorting to obtain a solution of alive and single Nr5a1-GFP+ cells. RNA was extracted from these cells with RNeasy Mini kit (Qiagen).
Sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold (Ribo-Zero), with 3 replicates by condition.
Sequencing with Illumina HiSeq 2500 (50 bp, paired-end)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Sex: XY
bulk RNA sequencing Leydig cells
FLcs_vs_ALCs_DESEq2_results.csv
|
| Data processing |
Reads were demultiplexed with Casava (v1.8.2)
Mapped with GemTools (v1.7.1)
Read counts and RPKM gene expression quantifications were calculated with an in-house pipeline based on Gencode annotation GRCm38 (v4)
Genome_build: GRCm38 (mm10)
Supplementary_files_format_and_content: counts and rpkm matrices
Supplementary_files_format_and_content: rMATS (splicing events) output
|
| |
|
| Submission date |
Apr 08, 2021 |
| Last update date |
Jun 29, 2021 |
| Contact name |
Serge Nef |
| E-mail(s) |
serge.nef@unige.ch
|
| Organization name |
University of Geneva
|
| Department |
Genetic Medicine and Development
|
| Street address |
Rue Michel-Servet 1
|
| City |
Genève |
| ZIP/Postal code |
1206 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE171746 |
Bulk RNA-seq of fetal and adult murine Leydig cells |
|
| Relations |
| BioSample |
SAMN18677467 |
| SRA |
SRX10559531 |
