|
| Status |
Public on Jun 30, 2022 |
| Title |
ChIP-seq_E16.5_H3K9ac-rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Telencephalon
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
age: Embronic day 16.5
tissue: telencephalon
antibody(vendor): PTM Biolab, Hangzhou, China
antibody(catalog#): PTM-156
antibody(lot#): 212620242H316
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Standard Native Chromatin Immunoprecipitation (N-ChIP) was employed to extract chromatin and perform immunoprecipitation, the IPed DNA was extracted by the phenol-chloroform method.
ChIP’s DNA was subjected to end-repair and then was 3’adenylated. Adaptors were ligated to the ends of these 4’adenylated fragments. Next, polymerase chain reaction amplification was to amplify those fragments with adaptors from previous step.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
genome_build: GRCm38
ChIP-seq reads were aligned to the mouse GRCm38 reference genome (vM23 from GENCODE) using Bowtie 2 (v.2.4.1) with default parameters. MACS (v.2.2.7.1) was used to call peaks (-q 0.01) relative to the input sample.
|
| |
|
| Submission date |
Mar 29, 2021 |
| Last update date |
Jun 30, 2022 |
| Contact name |
Dai Shang Kun |
| E-mail(s) |
daisk@sdut.edu.cn
|
| Organization name |
Shandong University of Technology
|
| Department |
School of Life Sciences and Medicine
|
| Lab |
Laboratory of Developmental and Evolutionary Biology
|
| Street address |
266 Xincun West Road
|
| City |
Zibo |
| State/province |
Shandong |
| ZIP/Postal code |
255000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE171088 |
Dynamic profiling of histone Kcr and Kla during neural differentiation in vivo |
|
| Relations |
| BioSample |
SAMN18527987 |
| SRA |
SRX10469189 |
