GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM518135 Query DataSets for GSM518135
Status Public on Mar 05, 2010
Title U373 CC3 20J/m2 UV treated and left to recover for 2 hours, Biological replicate 1, technical replicate 3
Sample type RNA
Source name U373 with constitutive CC3 expression, Treated with 20J/m2 UV, allowed to recover for 2 hr.
Organism Homo sapiens
Characteristics cell type: glioblastoma cell line
cell line: U373
Extracted molecule total RNA
Extraction protocol . Total RNA was isolated using RNeasy Mini Kit (Qiagen).
Label Cy5
Label protocol Five mg of total RNA was used for Cy5-labeled aRNA preparation per manufacturer instruction (Ambion MessageAmp™ aRNA Amplification Kit, Ambion Inc., Austn, TX, USA).   
Hybridization protocol Detailed descriptions of the gene array list, hybridization and processing procedures are available from
Scan protocol The arrays were then scanned using GenePix 4000B (Molecular Devices, Sunnyvale, CA, USA) and the fluorescent intensities are extracted from the generated images by following the instructions and the conditions described in HOA User Guide. The raw intensity data were input to GeneSpring GX Version 7.3.1 (Agilent, Foster City, CA, USA).
Description H57-0603008528 580 33 460 10 10_0635
Data processing The raw intensity data were input to GeneSpring GX Version 7.3.1 (Agilent, Foster City, CA, USA). The averaged intensities for each probe then were normalized by quantile normalization. Differentially expressed genes were identified and filtered based on t-tests (p< 0.05) and fold-change cutoffs of 1.7 fold.
Submission date Mar 04, 2010
Last update date Mar 04, 2010
Contact name Sylvia Fong
Phone 510-420-4167
Organization name Bionovo Inc
Department Biology
Street address 5858 Horton St Suite 400
City Emeryville
State/province CA
ZIP/Postal code 94608
Country USA
Platform ID GPL6254
Series (1)
GSE20633 Exogenous expression of CC3/TIP30 in U373 cells with or without UV treatment

Data table header descriptions
VALUE Quantile normalized signal intensity

Data table
PH_hs_0000004 2627.01175
PH_hs_0000007 1563.127596
PH_hs_0000008 5293.762039
PH_hs_0000009 682.1294382
PH_hs_0000010 34276.62352
PH_hs_0000011 152.3606965
PH_hs_0000012 179.0673657
PH_hs_0000013 417.0287782
PH_hs_0000018 2158.225216
PH_hs_0000019 512.3123163
PH_hs_0000020 53193.08396
PH_hs_0000021 269.9360174

Total number of rows: 30968

Table truncated, full table size 584 Kbytes.

Supplementary file Size Download File type/resource
GSM518135.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap