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Sample GSM518133 Query DataSets for GSM518133
Status Public on Mar 05, 2010
Title U373 CC3 20J/m2 UV treated and left to recover for 2 hours, Biological replicate 1, technical replicate 1
Sample type RNA
Source name U373 with constitutive CC3 expression, Treated with 20J/m2 UV, allowed to recover for 2 hr.
Organism Homo sapiens
Characteristics cell type: glioblastoma cell line
cell line: U373
Extracted molecule total RNA
Extraction protocol . Total RNA was isolated using RNeasy Mini Kit (Qiagen).
Label Cy5
Label protocol Five mg of total RNA was used for Cy5-labeled aRNA preparation per manufacturer instruction (Ambion MessageAmp™ aRNA Amplification Kit, Ambion Inc., Austn, TX, USA).   
Hybridization protocol Detailed descriptions of the gene array list, hybridization and processing procedures are available from
Scan protocol The arrays were then scanned using GenePix 4000B (Molecular Devices, Sunnyvale, CA, USA) and the fluorescent intensities are extracted from the generated images by following the instructions and the conditions described in HOA User Guide. The raw intensity data were input to GeneSpring GX Version 7.3.1 (Agilent, Foster City, CA, USA).
Description H55-0603008530 580 33 460 10 10_0635
Data processing The raw intensity data were input to GeneSpring GX Version 7.3.1 (Agilent, Foster City, CA, USA). The averaged intensities for each probe then were normalized by quantile normalization. Differentially expressed genes were identified and filtered based on t-tests (p< 0.05) and fold-change cutoffs of 1.7 fold.
Submission date Mar 04, 2010
Last update date Mar 04, 2010
Contact name Sylvia Fong
Phone 510-420-4167
Organization name Bionovo Inc
Department Biology
Street address 5858 Horton St Suite 400
City Emeryville
State/province CA
ZIP/Postal code 94608
Country USA
Platform ID GPL6254
Series (1)
GSE20633 Exogenous expression of CC3/TIP30 in U373 cells with or without UV treatment

Data table header descriptions
VALUE Quantile normalized signal intensity

Data table
PH_hs_0000004 3260.128342
PH_hs_0000007 1347.252218
PH_hs_0000008 4320.287495
PH_hs_0000009 744.3688207
PH_hs_0000010 32902.2123
PH_hs_0000011 112.8287976
PH_hs_0000012 140.1839669
PH_hs_0000013 353.3676784
PH_hs_0000018 1900.230583
PH_hs_0000019 574.4130244
PH_hs_0000020 61595.96687
PH_hs_0000021 195.1760234

Total number of rows: 30968

Table truncated, full table size 584 Kbytes.

Supplementary file Size Download File type/resource
GSM518133.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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