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Sample GSM518131 Query DataSets for GSM518131
Status Public on Mar 05, 2010
Title U373 Neo Untreated Biological replicate 3, technical replicate 2
Sample type RNA
 
Source name U373 Neo control, untreated
Organism Homo sapiens
Characteristics cell type: glioblastoma cell line
cell line: U373
Extracted molecule total RNA
Extraction protocol . Total RNA was isolated using RNeasy Mini Kit (Qiagen).
Label Cy5
Label protocol Five mg of total RNA was used for Cy5-labeled aRNA preparation per manufacturer instruction (Ambion MessageAmp™ aRNA Amplification Kit, Ambion Inc., Austn, TX, USA).   
 
Hybridization protocol Detailed descriptions of the gene array list, hybridization and processing procedures are available from http://www.phalanxbiotech.com/Support/Downloads.html
Scan protocol The arrays were then scanned using GenePix 4000B (Molecular Devices, Sunnyvale, CA, USA) and the fluorescent intensities are extracted from the generated images by following the instructions and the conditions described in HOA User Guide. The raw intensity data were input to GeneSpring GX Version 7.3.1 (Agilent, Foster City, CA, USA).
Description H53-0603008529 580 33 460 10 10_0635
Data processing The raw intensity data were input to GeneSpring GX Version 7.3.1 (Agilent, Foster City, CA, USA). The averaged intensities for each probe then were normalized by quantile normalization. Differentially expressed genes were identified and filtered based on t-tests (p< 0.05) and fold-change cutoffs of 1.7 fold.
 
Submission date Mar 04, 2010
Last update date Mar 04, 2010
Contact name Sylvia Fong
E-mail(s) sylviaf@bionovo.com
Phone 510-420-4167
URL http://www.bionovo.com
Organization name Bionovo Inc
Department Biology
Street address 5858 Horton St Suite 400
City Emeryville
State/province CA
ZIP/Postal code 94608
Country USA
 
Platform ID GPL6254
Series (1)
GSE20633 Exogenous expression of CC3/TIP30 in U373 cells with or without UV treatment

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity

Data table
ID_REF VALUE
PH_hs_0000002
PH_hs_0000003
PH_hs_0000004 4705.974551
PH_hs_0000005
PH_hs_0000006
PH_hs_0000007 1528.06754
PH_hs_0000008 4091.075111
PH_hs_0000009 2691.608415
PH_hs_0000010 22953.51029
PH_hs_0000011
PH_hs_0000012 183.7309983
PH_hs_0000013 791.0776842
PH_hs_0000014
PH_hs_0000015
PH_hs_0000016
PH_hs_0000017
PH_hs_0000018 2705.573695
PH_hs_0000019 1959.70676
PH_hs_0000020 48882.38143
PH_hs_0000021 204.8194984

Total number of rows: 30968

Table truncated, full table size 584 Kbytes.




Supplementary file Size Download File type/resource
GSM518131.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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