GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM5174464 Query DataSets for GSM5174464
Status Public on Jan 11, 2023
Title CM-Ctl-1
Sample type RNA
Source name adult cardiomyocytes after PBS treatment for 2 days
Organism Mus musculus
Characteristics gender: male
age: 10-week-old
treatment: PBS for 2 days
Treatment protocol Doxycycline is intraperitoneal injection to induce cardiomyocyte reprogramming in vivo.
Growth protocol αMHC promoter-driven Yamanaka's OSKM factor mice was generated from cross-breeding with αMHC promoter-driven CRE mice and tetO-Yamanaka's OSKM factor mice
Extracted molecule total RNA
Extraction protocol After PBS or doxycycline treatment for 2 days, adult cardiomyocytes were isolated and RNA was extracted for microarray analysis
Label Cy3
Label protocol Low Input Quick-Amp Labeling kit (Agilent Technologies, USA) and labeled with Cy3 (CyDye, Agilent Technologies, USA) during the in vitro transcription process.
Hybridization protocol 1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
Description gene expression
Data processing Genespring sofware
Submission date Mar 16, 2021
Last update date Jan 11, 2023
Contact name Yuan-Yuan Cheng
Organization name Academia Sinica
Street address Academia road
City Taipei
ZIP/Postal code 11529
Country Taiwan
Platform ID GPL10333
Series (1)
GSE169035 Gene expressional changes in control or adult reprogramming cardiomyocytes

Data table header descriptions
VALUE Normalized signal intensity

Data table
12 17.255
13 5.60034
14 817.268
15 -3.2133
16 174.67
17 1763.78
18 2.73086
19 1.45904
20 214.96
21 33.4524
22 16.1857
23 2173.14
24 49.4689
25 43.0176
26 28.6216
27 327.977
28 12.9343
30 319.678
31 631.91
32 3.06505

Total number of rows: 43585

Table truncated, full table size 592 Kbytes.

Supplementary file Size Download File type/resource
GSM5174464_US93703722_252665516036_CM-Ctl-1_1_1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap