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Status |
Public on Jan 11, 2023 |
Title |
CM-Ctl-1 |
Sample type |
RNA |
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Source name |
adult cardiomyocytes after PBS treatment for 2 days
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Organism |
Mus musculus |
Characteristics |
gender: male age: 10-week-old treatment: PBS for 2 days
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Treatment protocol |
Doxycycline is intraperitoneal injection to induce cardiomyocyte reprogramming in vivo.
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Growth protocol |
αMHC promoter-driven Yamanaka's OSKM factor mice was generated from cross-breeding with αMHC promoter-driven CRE mice and tetO-Yamanaka's OSKM factor mice
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Extracted molecule |
total RNA |
Extraction protocol |
After PBS or doxycycline treatment for 2 days, adult cardiomyocytes were isolated and RNA was extracted for microarray analysis
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Label |
Cy3
|
Label protocol |
Low Input Quick-Amp Labeling kit (Agilent Technologies, USA) and labeled with Cy3 (CyDye, Agilent Technologies, USA) during the in vitro transcription process.
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Hybridization protocol |
1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
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Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
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Description |
gene expression
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Data processing |
Genespring sofware
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Submission date |
Mar 16, 2021 |
Last update date |
Jan 11, 2023 |
Contact name |
Yuan-Yuan Cheng |
E-mail(s) |
nataschayuanyuan@gmail.com
|
Organization name |
Academia Sinica
|
Street address |
Academia road
|
City |
Taipei |
ZIP/Postal code |
11529 |
Country |
Taiwan |
|
|
Platform ID |
GPL10333 |
Series (1) |
GSE169035 |
Gene expressional changes in control or adult reprogramming cardiomyocytes |
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