|
Status |
Public on Aug 05, 2021 |
Title |
retina, CTRL |
Sample type |
SRA |
|
|
Source name |
retina
|
Organism |
Macaca fascicularis |
Characteristics |
disease state: control tissue: retina
|
Extracted molecule |
polyA RNA |
Extraction protocol |
All retina tissues were digested at 37℃, and were dissociated and triturated into single cell suspensions with 0.04% bovine serum albumin (BSA) in PBS solution. Single cell suspensions were diluted at a concentration of 500-1800 cells/uL in 0.04% BSA/Ames. 10x Genomics Chromium single cell system with v2
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
Cellranger v3.1 was used for demultiplexing, aligment and quantification. Genome_build: Macaca_fascicularis_5.0 Supplementary_files_format_and_content: h5 file
|
|
|
Submission date |
Mar 15, 2021 |
Last update date |
Aug 05, 2021 |
Contact name |
yuhua xiao |
E-mail(s) |
xiaoyh26@mail2.sysu.edu.cn
|
Phone |
18728192414
|
Organization name |
Sun Yat-sen University
|
Department |
zhongshan Ophthalmic center
|
Street address |
jinsui load
|
City |
guangzhou |
State/province |
guangdong province |
ZIP/Postal code |
510627 |
Country |
China |
|
|
Platform ID |
GPL23096 |
Series (1) |
GSE168908 |
Single-cell transcriptome profiling reveals the suppressive role of retinal neurons in microglia activation under diabetes mellitus |
|
Relations |
BioSample |
SAMN18311289 |
SRA |
SRX10341669 |