|
| Status |
Public on Mar 12, 2024 |
| Title |
Normal 2 |
| Sample type |
genomic |
| |
|
| Source name |
Patient
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tissue: HPV negative normal cervical epithelium
|
| Growth protocol |
Primary uterine cervical lesions and normal ectocervix were collected from the hospital section of Chittaranjan National Cancer Institute, Kolkata, India after appropriate approval of the Institutional Ethical Committee and written informed consent from respective patients were taken.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA from cryosections (5µm) of microdissected tumor tissue specimen and ectocervix were isolated by standard phenol/chloroform method.
|
| Label |
biotin
|
| Label protocol |
500ng of DNA are used for bisulfite conversion using the Zymo Research EZ-96 DNA Methylation Kit and labelling was done by standard Illumina Protocol using biotin
|
| |
|
| Hybridization protocol |
The bisulfite converted DNA extracts were hybridized to Infinium EPIC BeadChips using standard Illumina Protocol
|
| Scan protocol |
Arrays were imaged using iScan System using standard recommended Illumina scanner setting
|
| Description |
SAMPLE 12
|
| Data processing |
GenomeStudio v2011.1 (Methylation Module 1.9.0) was used to transform the data. No normalisation was performed.
|
| |
|
| Submission date |
Mar 12, 2021 |
| Last update date |
Mar 12, 2024 |
| Contact name |
Susanta Roychoudhury |
| E-mail(s) |
anirbanr91@gmail.com
|
| Organization name |
Saroj Gupta Cancer Centre & Research Institute
|
| Street address |
Mahatma Gandhi Road, Thakurpukur
|
| City |
Kolkata |
| State/province |
West Bengal |
| ZIP/Postal code |
700063 |
| Country |
India |
| |
|
| Platform ID |
GPL23976 |
| Series (1) |
| GSE168841 |
Genome-wide methylation profile of uterine cervical carcinoma in Indian patients |
|
