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Sample GSM515256 Query DataSets for GSM515256
Status Public on Jun 02, 2010
Title HeLa_Control (Cy5)_HeLa_MALAT-1 (Cy3)_Replication 2
Sample type RNA
 
Channel 1
Source name HeLa MALAT-1 Cy3
Organism Homo sapiens
Characteristics cell line: HeLa TO cells
protocol: MALAT-1 knockdown
sirna: stealth MALAT483
Treatment protocol To carry out RNA interference, siRNA duplexes, synthesized by SIGMA or Invitrogen were transfected into cells using Lipofectamine RNAi MAX (Invitrogen) according to the manufacturer's guidelines
Growth protocol Cells were grown at 37°C, 5% CO2 in Dulbecco’s Modified Eagle's Medium supplemented with 10% fetal calf serum and penicillin/streptomycin
Extracted molecule total RNA
Extraction protocol Invitrogen Trizol reagent
Label Cy3
Label protocol Low RNA Input Linear Amplification kit standard protocol
 
Channel 2
Source name HeLa Control-1 Cy5
Organism Homo sapiens
Characteristics cell line: HeLa TO cells
protocol: control
Treatment protocol Control
Growth protocol Cells were grown at 37°C, 5% CO2 in Dulbecco’s Modified Eagle's Medium supplemented with 10% fetal calf serum and penicillin/streptomycin
Extracted molecule total RNA
Extraction protocol Invitrogen Trizol reagent
Label Cy5
Label protocol Low RNA Input Linear Amplification kit standard protocol
 
 
Hybridization protocol Hybridization_Kit: Agilent in situ hybridization plus
Scan protocol Scanner: Agilent DNA microarray Scaner
Description Mammalian non-coding transcript
Hela Malat 126-4
Data processing Agilent Feature Extraction ver 9.5, normalized (lowess and rank consistency)
 
Submission date Feb 24, 2010
Last update date Jun 01, 2010
Contact name RANDEEP RAKWAL
E-mail(s) plantproteomics@gmail.com
Phone +81-(0)90-1853-7875
Organization name University of Tsukuba
Department Institute of Health and Sport Sciences
Lab GSI 403
Street address 1-1-1 Tennodai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8574
Country Japan
 
Platform ID GPL4133
Series (1)
GSE20506 Identification of cis- and trans-acting factors involved in the localization of MALAT-1 to nuclear speckles

Data table header descriptions
ID_REF
VALUE Log 10 based (normalized ch1intensity / normalized ch2 intensity)
Norm_Ch1 Normalized (lowess and rank consistency) signal of ch1
Norm_Ch2 Normalized (lowess and rank consistency) signal of ch2
Raw_Ch1 The net CH1 signal following the subtraction of the background from the raw mean CH1 signal
Raw_Ch2 The net CH2 signal following the subtraction of the background from the raw mean CH2 signal

Data table
ID_REF VALUE Norm_Ch1 Norm_Ch2 Raw_Ch1 Raw_Ch2
1 -0.12 123559.1 92889.44 74975.3 461823
2 -0.58 7.98 2.09 6.17 -3.86
3 -0.73 11.02 2.06 9.7 -7.24
4 -0.7 10.27 2.07 9.01 -0.58
5 -0.71 10.55 2.07 9.19 -8.99
6 -0.68 9.85 2.07 8.56 -0.94
7 -0.69 10.21 2.07 8.81 -6.51
8 -0.81 13.33 2.05 11.33 -7.6
9 -0.75 11.49 2.06 9.78 -4.6
10 -0.6 8.35 2.09 4.6 1.21
11 -0.6 8.38 2.09 3.13 -7.47
12 0.06 479 550.24 361.61 2068.29
13 -0.2 235.59 149.32 174.09 571.9
14 -0.09 171.06 139.46 125.46 536.74
15 -0.25 20.08 11.32 15.35 41.71
16 -0.11 27565.03 21640.63 18646.4 89896
17 -0.06 31.34 27.57 22.8 106.28
18 -0.28 316.5 166.04 232.25 633.22
19 0.1 64713.88 81265.49 38257.1 383944
20 -0.44 37.49 13.69 27.58 51.86

Total number of rows: 45015

Table truncated, full table size 1652 Kbytes.




Supplementary file Size Download File type/resource
GSM515256_US22502699_251485029126_S01_GE2_v5_95_Feb07_126_4.txt.gz 15.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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