|
| Status |
Public on Jul 22, 2021 |
| Title |
wt.3w.rep3 |
| Sample type |
SRA |
| |
|
| Source name |
WT left ventricle 3w
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
genotype: wt
age: 3w
surgery: none
tissue: left ventricle
|
| Treatment protocol |
For ischemic heart disease models, 12-week-old male C57Bl6/J mice were randomized to temporary (ischemia-reperfusion; I/R) or permanent (myocardial infarction; MI) ligation of the left anterior descending (LAD) coronary artery or sham surgery. Three days post-I/R or 8 weeks post-MI, left ventricular (LV) tissues were collected from the area remote of the infarcted myocardium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard Trizol total RNA extraction
TruSeq mRNA stranded (illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
kb03
|
| Data processing |
aligment - STAR 2.7.3a
readcount analysis - featurecounts (SubRead 2.0.0)
Genome_build: mm10
Supplementary_files_format_and_content: PLN_featureCounts_raw.txt (raw readcounts)
|
| |
|
| Submission date |
Mar 10, 2021 |
| Last update date |
Jul 22, 2021 |
| Contact name |
Cornelis J. Boogerd |
| E-mail(s) |
k.boogerd@hubrecht.eu
|
| Organization name |
Hubrecht Institute
|
| Department |
Developmental Biology and Stem Cell Research
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE168610 |
Transcriptomic analyses of cardiomyopathy development in mice carrying the phospholamban p.(Arg14del) pathogenic variant, and comparison with ischemic heart disease |
|
| Relations |
| BioSample |
SAMN18238337 |
| SRA |
SRX10302614 |
