|
| Status |
Public on Feb 20, 2024 |
| Title |
FGK45_5 |
| Sample type |
SRA |
| |
|
| Source name |
draining lymph nodes
|
| Organism |
Mus musculus |
| Characteristics |
agent: FGK45.5
strain: C57BL/6
group: tumor-bearing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Draining lymph nodes were removed and CD19+ B cells were sorted and collected by FACS. Flash frozen on dry ice, and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
tumor-bearing mice injected with FGK45.5
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Reads were aligned to the reference mouse genome (version GRCm38) using HISAT2 (version 2.0.5)
expression levels were calculated as Fragments per Kilobase of transcript per Million reads(FPKM) using StringTie (version 1.3.3b)
Genome_build: GRCm38
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Mar 09, 2021 |
| Last update date |
Feb 20, 2024 |
| Contact name |
Cong Fu |
| Organization name |
The First Hospital of Jilin University
|
| Lab |
Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education
|
| Street address |
NO.17 Xinmin Street
|
| City |
Changchun |
| State/province |
Jilin |
| ZIP/Postal code |
130000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE168523 |
PPARĪ“ inhibition blocks tumor-induced Bregs and enhances CD40 agonist immunotherapy |
|
| Relations |
| BioSample |
SAMN18221276 |
| SRA |
SRX10291240 |
