|
Status |
Public on Mar 04, 2024 |
Title |
Ovary-VC treated- rep1 |
Sample type |
SRA |
|
|
Source name |
ovary 16h post-hCG
|
Organism |
Mus musculus |
Characteristics |
strain: CBA x C57BL/6 F1 age: 21days old; 10-12 grams treatment: 7.5% DMSO
|
Treatment protocol |
mice were injected i.p either with CRS or 7.5% DMSO vehicle on days 21, 22, 23 and 24
|
Growth protocol |
21 days old mice; 10-12grams
|
Extracted molecule |
total RNA |
Extraction protocol |
one ovary from each mice was processed for RNA extraction using RNeasy Mini Kit (Qiagen) and Dnase-treated. Total RNA was converted to strand specific Illumina compatible sequencing libraries using the Nugen Universal Plus mRNA mRNA-Seq library kit from Tecan (Mannedorf, Switzerland) as per the manufacturers instructions (MO1442 v2). 75bp SR sequencing using Illumina NextSeq500 High output mode and v2.5 chemistry. Illumina Protocol 15069765 v06, July 2019.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
mice were injected i.p eCG on day 22 and hCG on day 24
|
Data processing |
FASTQ files checked with FastQC. Quasi-alignment to GRCm38.p6 (M25) transcript assembly and count quanticiation using Salmon. Differential expression analyses were performed using DESeq2. Genome_build: Genome Reference Consortium Mouse Build 38 patch release 6 Supplementary_files_format_and_content: Tabular list of raw and normalised (TPM) counts generated from Salmon
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|
|
Submission date |
Mar 05, 2021 |
Last update date |
Mar 04, 2024 |
Contact name |
Thao Dinh |
E-mail(s) |
doan.dinh@adelaide.edu.au
|
Organization name |
University of Adelaide
|
Street address |
North Terrace
|
City |
Adelaide |
State/province |
South Australia |
ZIP/Postal code |
5000 |
Country |
Australia |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE168348 |
Gene expression analyses of whole ovaries from mice that were treated with N-Cadherin antagonist CRS-006 |
|
Relations |
BioSample |
SAMN18153606 |
SRA |
SRX10244259 |