|
| Status |
Public on Mar 04, 2024 |
| Title |
COCs-VC treated- rep3 |
| Sample type |
SRA |
| |
|
| Source name |
COCs 10h IVM
|
| Organism |
Mus musculus |
| Characteristics |
strain: CBA x C57BL/6 F1
age: 21days old; 10-12 grams
treatment: 0.1% DMSO
|
| Treatment protocol |
COCs were treated either with CRS or 0.1% DMSO vehicle for 10h during in vitro maturaion assay
|
| Growth protocol |
COCs from 21 days old mice; 10-12grams
|
| Extracted molecule |
total RNA |
| Extraction protocol |
100 pooled COCs per treatment were processed for RNA extraction using RNeasy Mini Kit (Qiagen) and Dnase-treated.
Total RNA was converted to strand specific Illumina compatible sequencing libraries using the Nugen Universal Plus mRNA mRNA-Seq library kit from Tecan (Mannedorf, Switzerland) as per the manufacturers instructions (MO1442 v2). 75bp SR sequencing using Illumina NextSeq500 High output mode and v2.5 chemistry. Illumina Protocol 15069765 v06, July 2019.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
mice were injected i.p eCG on day 22
|
| Data processing |
FASTQ files checked with FastQC.
Quasi-alignment to GRCm38.p6 (M25) transcript assembly and count quanticiation using Salmon.
Differential expression analyses were performed using DESeq2.
Genome_build: Genome Reference Consortium Mouse Build 38 patch release 6
Supplementary_files_format_and_content: Tabular list of raw and normalised (TPM) counts generated from Salmon
|
| |
|
| Submission date |
Mar 05, 2021 |
| Last update date |
Mar 04, 2024 |
| Contact name |
Thao Dinh |
| E-mail(s) |
doan.dinh@adelaide.edu.au
|
| Organization name |
University of Adelaide
|
| Street address |
North Terrace
|
| City |
Adelaide |
| State/province |
South Australia |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE168347 |
Gene expression analyses of COCs treated with N-Cadherin antagonist CRS-006 |
|
| Relations |
| BioSample |
SAMN18153051 |
| SRA |
SRX10244254 |
