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Sample GSM5124081 Query DataSets for GSM5124081
Status Public on Sep 29, 2021
Title HT10L: Tibetan placenta miRNA
Sample type RNA
 
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: Tibetan
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.Total RNA from placentas was purified using mirVanaTM miRNA Isolation Kits and quantified.
Label biotin
Label protocol Starting from Total RNA, poly A tail was attached to the 3 'end of the small RNA under the guidance of poly A polymerase. Under the guidance of ligase, biotin labeled patented 3DNA Dendrimer was attached to the poly A tail of RNA.
 
Hybridization protocol Before using 20X Eukaryotic Hybridization Controls for the first time, confirm whether denatured at 65℃ for 5min has been performed. The hybridization solution was incubated in a constant temperature metal bath at 99°C for 5 min, and then 45°C for 5 min. The hybrid solution was removed from the thermostatic metal bath and centrifuged for 5 min at 13200rpm in a miniature centrifuge to remove insoluble substances in the hybrid mixture. Hybridized was preformed in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 48℃.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description miRNA expression data from Tibetan placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals
 
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
E-mail(s) tanna.mgl@gmail.com
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
 
Platform ID GPL19117
Series (2)
GSE168028 miRNA expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
ID_REF
VALUE Values presented are RMA signal intensity.

Data table
ID_REF VALUE
MIMAT0000001_st 770.0728
MIMAT0015091_st 2.652787
MIMAT0000002_st 2.430507
MIMAT0015092_st 2.12148
MIMAT0020301_st 2.060039
MIMAT0000003_st 1.934997
MIMAT0020302_st 2.791575
MIMAT0000004_st 1.812764
MIMAT0000005_st 2.506241
MIMAT0015093_st 2.595341
MIMAT0020303_st 1.976339
MIMAT0000006_st 2.155976
MIMAT0020304_st 1.93914
MIMAT0000007_st 2.107832
MIMAT0015094_st 2.332424
MIMAT0000008_st 1.955599
MIMAT0020305_st 1.868163
MIMAT0000009_st 2.576706
MIMAT0020306_st 1.86642
MIMAT0000010_st 1.880504

Total number of rows: 36249

Table truncated, full table size 874 Kbytes.




Supplementary file Size Download File type/resource
GSM5124081_150319-150225A_HT10L_miRNA-4_0.CEL.gz 796.8 Kb (ftp)(http) CEL
Processed data included within Sample table

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