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Sample GSM5124071 Query DataSets for GSM5124071
Status Public on Sep 29, 2021
Title P69: European placenta miRNA
Sample type RNA
 
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: European
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.Total RNA from placentas was purified using mirVanaTM miRNA Isolation Kits and quantified.
Label biotin
Label protocol Starting from Total RNA, poly A tail was attached to the 3 'end of the small RNA under the guidance of poly A polymerase. Under the guidance of ligase, biotin labeled patented 3DNA Dendrimer was attached to the poly A tail of RNA.
 
Hybridization protocol Before using 20X Eukaryotic Hybridization Controls for the first time, confirm whether denatured at 65℃ for 5min has been performed. The hybridization solution was incubated in a constant temperature metal bath at 99°C for 5 min, and then 45°C for 5 min. The hybrid solution was removed from the thermostatic metal bath and centrifuged for 5 min at 13200rpm in a miniature centrifuge to remove insoluble substances in the hybrid mixture. Hybridized was preformed in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 48℃.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description miRNA expression data from European placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals
 
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
E-mail(s) tanna.mgl@gmail.com
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
 
Platform ID GPL19117
Series (2)
GSE168028 miRNA expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
ID_REF
VALUE Values presented are RMA signal intensity.

Data table
ID_REF VALUE
MIMAT0000001_st 1337.538
MIMAT0015091_st 1.909513
MIMAT0000002_st 2.428987
MIMAT0015092_st 2.175831
MIMAT0020301_st 2.424681
MIMAT0000003_st 2.298197
MIMAT0020302_st 2.70997
MIMAT0000004_st 1.781966
MIMAT0000005_st 1.9719
MIMAT0015093_st 3.096174
MIMAT0020303_st 2.091975
MIMAT0000006_st 2.152572
MIMAT0020304_st 1.925365
MIMAT0000007_st 2.442968
MIMAT0015094_st 2.593701
MIMAT0000008_st 2.08234
MIMAT0020305_st 1.978419
MIMAT0000009_st 2.567388
MIMAT0020306_st 1.908471
MIMAT0000010_st 2.315203

Total number of rows: 36249

Table truncated, full table size 874 Kbytes.




Supplementary file Size Download File type/resource
GSM5124071_150319-150225A_P69_miRNA-4_0.CEL.gz 805.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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