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Sample GSM5123975 Query DataSets for GSM5123975
Status Public on Sep 29, 2021
Title HT10L: Tibetan placenta mRNA
Sample type RNA
 
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: Tibetan
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from Tibetan placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
 
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
E-mail(s) tanna.mgl@gmail.com
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
 
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
ID_REF
VALUE Values presented are RMA signal intensity.

Data table
ID_REF VALUE
AFFX-BioB-5_at 150.5729
AFFX-BioB-M_at 206.0626
AFFX-BioB-3_at 152.0232
AFFX-BioC-5_at 398.9574
AFFX-BioC-3_at 545.4106
AFFX-BioDn-5_at 1122.9100
AFFX-BioDn-3_at 2342.1660
AFFX-CreX-5_at 5834.5260
AFFX-CreX-3_at 6648.3390
AFFX-DapX-5_at 34.7934
AFFX-DapX-M_at 252.0274
AFFX-DapX-3_at 737.5705
AFFX-LysX-5_at 35.0224
AFFX-LysX-M_at 60.3729
AFFX-LysX-3_at 147.1014
AFFX-PheX-5_at 60.9262
AFFX-PheX-M_at 63.4648
AFFX-PheX-3_at 120.1614
AFFX-ThrX-5_at 13.2240
AFFX-ThrX-M_at 58.2012

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.




Supplementary file Size Download File type/resource
GSM5123975_150106-140651B_HT10L_PrimeView.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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