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Sample GSM5123973 Query DataSets for GSM5123973
Status Public on Sep 29, 2021
Title HT8L: Tibetan placenta mRNA
Sample type RNA
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: Tibetan
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from Tibetan placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
VALUE Values presented are RMA signal intensity.

Data table
AFFX-BioB-5_at 126.7226
AFFX-BioB-M_at 163.6055
AFFX-BioB-3_at 142.9707
AFFX-BioC-5_at 337.8596
AFFX-BioC-3_at 515.5085
AFFX-BioDn-5_at 1009.7460
AFFX-BioDn-3_at 2313.9020
AFFX-CreX-5_at 5186.3970
AFFX-CreX-3_at 6508.9110
AFFX-DapX-5_at 25.7492
AFFX-DapX-M_at 166.7443
AFFX-DapX-3_at 558.4946
AFFX-LysX-5_at 25.0382
AFFX-LysX-M_at 46.7635
AFFX-LysX-3_at 103.7158
AFFX-PheX-5_at 45.8722
AFFX-PheX-M_at 43.0742
AFFX-PheX-3_at 87.3810
AFFX-ThrX-5_at 11.6708
AFFX-ThrX-M_at 43.5246

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.

Supplementary file Size Download File type/resource
GSM5123973_150106-140651B_HT8L_PrimeView.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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