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Sample GSM5123972 Query DataSets for GSM5123972
Status Public on Sep 29, 2021
Title HT7L: Tibetan placenta mRNA
Sample type RNA
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: Tibetan
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from Tibetan placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
VALUE Values presented are RMA signal intensity.

Data table
AFFX-BioB-5_at 157.0127
AFFX-BioB-M_at 210.8563
AFFX-BioB-3_at 183.9432
AFFX-BioC-5_at 413.1763
AFFX-BioC-3_at 602.8661
AFFX-BioDn-5_at 1144.3730
AFFX-BioDn-3_at 2313.2370
AFFX-CreX-5_at 5611.5240
AFFX-CreX-3_at 6728.5670
AFFX-DapX-5_at 33.7630
AFFX-DapX-M_at 211.0320
AFFX-DapX-3_at 597.8420
AFFX-LysX-5_at 27.9692
AFFX-LysX-M_at 61.0726
AFFX-LysX-3_at 125.2090
AFFX-PheX-5_at 57.6193
AFFX-PheX-M_at 51.6124
AFFX-PheX-3_at 110.5593
AFFX-ThrX-5_at 11.8092
AFFX-ThrX-M_at 51.1621

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.

Supplementary file Size Download File type/resource
GSM5123972_150106-140651B_HT7L_PrimeView.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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