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Sample GSM5123969 Query DataSets for GSM5123969
Status Public on Sep 29, 2021
Title HT4L: Tibetan placenta mRNA
Sample type RNA
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: Tibetan
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from Tibetan placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
VALUE Values presented are RMA signal intensity.

Data table
AFFX-BioB-5_at 156.2995
AFFX-BioB-M_at 208.8233
AFFX-BioB-3_at 162.8380
AFFX-BioC-5_at 377.0268
AFFX-BioC-3_at 555.4792
AFFX-BioDn-5_at 1138.3870
AFFX-BioDn-3_at 2238.3600
AFFX-CreX-5_at 5448.0660
AFFX-CreX-3_at 6169.3600
AFFX-DapX-5_at 31.7527
AFFX-DapX-M_at 209.1252
AFFX-DapX-3_at 640.4630
AFFX-LysX-5_at 33.8519
AFFX-LysX-M_at 67.1428
AFFX-LysX-3_at 145.8793
AFFX-PheX-5_at 64.0110
AFFX-PheX-M_at 58.1001
AFFX-PheX-3_at 115.9060
AFFX-ThrX-5_at 14.8000
AFFX-ThrX-M_at 54.4703

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.

Supplementary file Size Download File type/resource
GSM5123969_150106-140651B_HT4L_PrimeView.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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