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Sample GSM5123965 Query DataSets for GSM5123965
Status Public on Sep 29, 2021
Title P69: European placenta mRNA
Sample type RNA
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: European
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from European placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
VALUE Values presented are RMA signal intensity.

Data table
AFFX-BioB-5_at 244.4839
AFFX-BioB-M_at 354.3458
AFFX-BioB-3_at 244.0767
AFFX-BioC-5_at 656.4449
AFFX-BioC-3_at 863.3977
AFFX-BioDn-5_at 1828.0310
AFFX-BioDn-3_at 3497.3170
AFFX-CreX-5_at 7979.4170
AFFX-CreX-3_at 9261.4840
AFFX-DapX-5_at 39.8069
AFFX-DapX-M_at 460.9699
AFFX-DapX-3_at 1524.6340
AFFX-LysX-5_at 73.1073
AFFX-LysX-M_at 140.9704
AFFX-LysX-3_at 322.7856
AFFX-PheX-5_at 89.6440
AFFX-PheX-M_at 116.2469
AFFX-PheX-3_at 209.2117
AFFX-ThrX-5_at 13.5228
AFFX-ThrX-M_at 104.1930

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.

Supplementary file Size Download File type/resource
GSM5123965_150106-140651A_P69-1_PrimeView.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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