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Sample GSM5123964 Query DataSets for GSM5123964
Status Public on Sep 29, 2021
Title P67: European placenta mRNA
Sample type RNA
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: European
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from European placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
VALUE Values presented are RMA signal intensity.

Data table
AFFX-BioB-5_at 351.6344
AFFX-BioB-M_at 533.6643
AFFX-BioB-3_at 382.6629
AFFX-BioC-5_at 900.5282
AFFX-BioC-3_at 1232.4680
AFFX-BioDn-5_at 2639.7190
AFFX-BioDn-3_at 4660.3450
AFFX-CreX-5_at 9410.4530
AFFX-CreX-3_at 11106.4800
AFFX-DapX-5_at 65.0102
AFFX-DapX-M_at 506.5651
AFFX-DapX-3_at 1619.8580
AFFX-LysX-5_at 65.4420
AFFX-LysX-M_at 143.0623
AFFX-LysX-3_at 294.3291
AFFX-PheX-5_at 103.4156
AFFX-PheX-M_at 111.6545
AFFX-PheX-3_at 217.0016
AFFX-ThrX-5_at 14.8914
AFFX-ThrX-M_at 94.1356

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.

Supplementary file Size Download File type/resource
GSM5123964_150106-140651A_P67-1_PrimeView.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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