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Sample GSM5123963 Query DataSets for GSM5123963
Status Public on Sep 29, 2021
Title P63: European placenta mRNA
Sample type RNA
 
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: European
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from European placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
 
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
E-mail(s) tanna.mgl@gmail.com
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
 
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
ID_REF
VALUE Values presented are RMA signal intensity.

Data table
ID_REF VALUE
AFFX-BioB-5_at 334.4644
AFFX-BioB-M_at 482.3979
AFFX-BioB-3_at 386.1704
AFFX-BioC-5_at 934.3535
AFFX-BioC-3_at 1250.994
AFFX-BioDn-5_at 2345.483
AFFX-BioDn-3_at 4557.826
AFFX-CreX-5_at 9111.211
AFFX-CreX-3_at 10788.9
AFFX-DapX-5_at 64.0039
AFFX-DapX-M_at 488.6541
AFFX-DapX-3_at 1733.425
AFFX-LysX-5_at 71.7172
AFFX-LysX-M_at 145.7092
AFFX-LysX-3_at 308.1745
AFFX-PheX-5_at 104.2594
AFFX-PheX-M_at 130.5335
AFFX-PheX-3_at 240.6647
AFFX-ThrX-5_at 15.1218
AFFX-ThrX-M_at 98.0855

Total number of rows: 49395

Table truncated, full table size 1036 Kbytes.




Supplementary file Size Download File type/resource
GSM5123963_150106-140651A_P63-1_PrimeView.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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