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Sample GSM5123959 Query DataSets for GSM5123959
Status Public on Sep 29, 2021
Title P55: European placenta mRNA
Sample type RNA
Source name placenta
Organism Homo sapiens
Characteristics gender: Female
tissue: Placenta
population: European
Extracted molecule total RNA
Extraction protocol Total RNA from placentas was isolated using RNeasy Mini Kits according to the manufacturer’s instructions. RNA quantity was determined using an Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol cRNA in hybridization solution was denatured at 99℃ for 5 min and 45℃ for 5 min prior to loading onto a microarray. Arrays were hybridized in a Agilent Hybridization Oven for 16hr at a rotation speed of 60 rpm at 45℃ and washed with Wash Buffer A and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanned on Affymetrix GeneChip Scanner 3000
Description Gene expression data from European placenta
Data processing AGCC Software (Affymetrix® GeneChip® Command Console® Software) converts.DAT files from image signals to digital signals known as.CEL files. CEL files record probe level fluorescent signals.
Submission date Mar 02, 2021
Last update date Sep 29, 2021
Contact name Tanna Wuren
Organization name Qinghai University
Street address Kunlun Road No.16
City xining
ZIP/Postal code 810001
Country China
Platform ID GPL15207
Series (2)
GSE168021 Gene expression in Placental Tissue from high altitude European and Tibetan women
GSE168130 miRNA and mRNA expression in placental tissue from high altitude pregnant women

Data table header descriptions
VALUE Values presented are RMA signal intensity.

Data table
AFFX-BioB-5_at 431.3149
AFFX-BioB-M_at 592.8091
AFFX-BioB-3_at 460.4337
AFFX-BioC-5_at 1128.0240
AFFX-BioC-3_at 1442.8220
AFFX-BioDn-5_at 3154.6290
AFFX-BioDn-3_at 5301.5690
AFFX-CreX-5_at 10304.9900
AFFX-CreX-3_at 10779.9900
AFFX-DapX-5_at 67.7892
AFFX-DapX-M_at 613.1672
AFFX-DapX-3_at 2302.4030
AFFX-LysX-5_at 83.7311
AFFX-LysX-M_at 197.9250
AFFX-LysX-3_at 411.1623
AFFX-PheX-5_at 126.3380
AFFX-PheX-M_at 153.1573
AFFX-PheX-3_at 279.1224
AFFX-ThrX-5_at 16.0391
AFFX-ThrX-M_at 138.3083

Total number of rows: 49395

Table truncated, full table size 1043 Kbytes.

Supplementary file Size Download File type/resource
GSM5123959_150106-140651A_P55-1_PrimeView.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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