|
| Status |
Public on Aug 24, 2021 |
| Title |
SJRHB015721_X1_shDBX1_A_Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
SJRHB015721_X1 FN-RMS cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SJRHB015721_X1
treatment: shDBX1-A
|
| Treatment protocol |
Cells were transduced with pLKO.3G.Scr or pLKO.3G.shDBX1-A (or B) overnight. The virus was removed the next morning. RNA was harvested 72 hours post-transduction.
|
| Growth protocol |
SJRHB015721_X1 cells are maintained in complete neurobasal medium as described in (Drummond et al., (2018) Cancer Cell).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA harvested with Qiagen't miRNeasy RNA protocol.
|
| Label |
biotin
|
| Label protocol |
Samples were labeled with the WT Plus Assay Kit (#902280, ThermoScientific) according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized with the WT Plus Assay Kit (#902280, ThermoScientific) according to manufacturer's protocol.
|
| Scan protocol |
Samples were scanned on a Gene Chip Scanner 3000 7G.
|
| Description |
MEH193
SJRHB015721_X1_shDBX1_A_R2
|
| Data processing |
The scanned images are then analyzed with Expression Console software (Affymetrix) to obtain raw data and metrics for Quality Controls. The distribution of raw data show no outlier experiment. Affymetrix probe-set data were normalized using the robust multi-array average (RMA) method.
|
| |
|
| Submission date |
Feb 16, 2021 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Hongjian Jin |
| E-mail(s) |
hongjian.jin@STJUDE.ORG
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Center for Applied Bioinformatics
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38015 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE166810 |
Transcriptomic changes in SJRHB015721_X1 human FN-RMS following DBX1 knock-down |
| GSE166906 |
Synthetic essentiality between PTEN and core dependency factor PAX7 dictates rhabdomyosarcoma identity |
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