|
| Status |
Public on Aug 24, 2021 |
| Title |
ASP cKO tumor rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Pten cKO tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: tumor
genotype: aP2-Cre;SmoM2;Pten fl/fl
|
| Treatment protocol |
Four wild-type tumors and four Pten cKO tumors.
|
| Growth protocol |
Tumors harvested when mice reached humane endpoints as determined by SJCRH IACUC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted with Qiagen's miRNeasy RNA extraction protocol according to manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated according to the GeneChip Mouse 2.0 ST array protocol
|
| |
|
| Hybridization protocol |
Hybridized according to the GeneChip Mouse 2.0 ST array protocol
|
| Scan protocol |
GeneChips were scanned using the affymetrix GCS3000 7G system
|
| Description |
SPA.190
MEH125_ASPcKO
|
| Data processing |
The scanned images are then analyzed with Expression Console software (Affymetrix) to obtain raw data and metrics for Quality Controls. The distribution of raw data show no outlier experiment. Affymetrix probe-set data were normalized using the robust multi-array average (RMA) method.
|
| |
|
| Submission date |
Feb 16, 2021 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Hongjian Jin |
| E-mail(s) |
hongjian.jin@STJUDE.ORG
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Center for Applied Bioinformatics
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38015 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE166809 |
Differences in gene expression between ASP WT and ASPcKO tumors |
| GSE166906 |
Synthetic essentiality between PTEN and core dependency factor PAX7 dictates rhabdomyosarcoma identity |
|
