Cynomologus macaque (Macaca fascicularis). To conserve animals, we acquired blood samples from NHPs already in use with other research protocols. Male cynomologus macaques (Covance, Denver, PA) were wild caught and of Vietnamese origin. In a separate experiment, these animals had been previously exposed to human butyrylcholinesterase and survived a 5.5xLD50 challenge of soman in December 2003. The animals were certified to be in good health and weighed 3-4 kg at the time of the blood collection (May 13, 2004). Whole blood tissue from each donor was collected using a 5 cc syringe and immediately injected into a PAXgene™ Blood RNA Collection Tube (PreAnalytiX, Franklin Lakes, NJ). Whole blood tissue was withdrawn from the right or left saphenous vein. Approximately 2.5 mL of whole blood tissue was obtained from the rhesus macaques. All samples were incubated in the PAXgene™ Blood RNA tube for 24 hrs at room temperature prior to RNA extraction. Keywords = cynomologus macaque Keywords = non-human primate Keywords = blood
