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Sample GSM5065186 Query DataSets for GSM5065186
Status Public on Feb 05, 2021
Title O_WT_370
Sample type SRA
Source name myeloid biased HSC
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: myeloid biased HSC
genotype: Igf2bp2 WT
age: 26 Months
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by using MagMax96 total RNA isolation kit (Ambion, AM1830) according to the manufacturer’s protocol.
RNA libraries were prepared for sequencing using Nextera XT DNA Library Preparation Kit
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing Total RNA sequenced reads were pseudoaligned to the mm10 reference cDNA using Salmon v1.4.0.
Single Cell RNA sequenced reads were aligned to the mm10 reference genome using 10X Genomics Cell Ranger v5.0.0.
Genome_build: mm10
Supplementary_files_format_and_content: Tar files with the output of Salmon, including quant.sf containing the TPM values
Supplementary_files_format_and_content: Tar files with raw and filtered gene matrices, features, and barcodes
Submission date Feb 04, 2021
Last update date Feb 05, 2021
Contact name Marthe Behrends
Organization name Leibniz Institute on Aging – Fritz Lipmann Institute
Lab Rudolph
Street address Beutenbergstraße 11
City Jena
ZIP/Postal code 07745
Country Germany
Platform ID GPL24247
Series (1)
GSE166176 Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC
BioSample SAMN17795404
SRA SRX10024528

Supplementary file Size Download File type/resource
GSM5065186_7_O_WT_370_S6.tar.gz 1.9 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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