|Public on Mar 10, 2021
|Humanized Blood Exosomes
|Homo sapiens; Mus musculus
|strain: FRG KO on C57BL/6
host organism: FRG KO mice
xenograft organism: Homo sapiens
sample type: exosome
|HepaCur™serum derived from FRG® KO mice were sent for exosomal RNA-sequencing analysis.
|FRG® KO on C57BI/6 humanized liver model mice are repopulated with human liver cells from multiple qualified donors.
|Exosome isolation was performed by System Biosciences with the ExoQuick Isolation & RNA Purification Kits
Exosome isolation and subsequent Exo-NGS exosomal RNA-sequencing were performed by System Biosciences.
|Illumina NextSeq 500
|Sequenced reads were trimmed using trimgalore
The trimmed reads were aligned to a combined human (b38) and mouse (mm10) genome using HISAT2 align with parameters --no-mixed --no-discordant
Aligned reads were transformed into .bam files and sorted by coordinate using sambamba view and sambamba sort.
Read counts were generated using the featureCounts program from the subread package.
Genome_build: GRCh38 and mm10
Supplementary_files_format_and_content: tab-delimited text files include reads counts for the sample
|Jan 22, 2021
|Last update date
|Mar 10, 2021
|Laboratory of Obesity and Metabolic Diseases
|9000 Rockville Pike
|Exosome derived from liver humanized mice