GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM5031952 Query DataSets for GSM5031952
Status Public on Mar 10, 2021
Title Humanized Blood Exosomes
Sample type SRA
Source name liver
Organisms Homo sapiens; Mus musculus
Characteristics strain: FRG KO on C57BL/6
host organism: FRG KO mice
xenograft organism: Homo sapiens
tissue: serum
sample type: exosome
Treatment protocol HepaCur™serum derived from FRG® KO mice were sent for exosomal RNA-sequencing analysis.
Growth protocol FRG® KO on C57BI/6 humanized liver model mice are repopulated with human liver cells from multiple qualified donors.
Extracted molecule total RNA
Extraction protocol Exosome isolation was performed by System Biosciences with the ExoQuick Isolation & RNA Purification Kits
Exosome isolation and subsequent Exo-NGS exosomal RNA-sequencing were performed by System Biosciences.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Sequenced reads were trimmed using trimgalore
The trimmed reads were aligned to a combined human (b38) and mouse (mm10) genome using HISAT2 align with parameters --no-mixed --no-discordant
Aligned reads were transformed into .bam files and sorted by coordinate using sambamba view and sambamba sort.
Read counts were generated using the featureCounts program from the subread package.
Genome_build: GRCh38 and mm10
Supplementary_files_format_and_content: tab-delimited text files include reads counts for the sample
Submission date Jan 22, 2021
Last update date Mar 10, 2021
Contact name Haiming Cao
Phone 3014029032
Organization name NIH
Department NHLBI
Lab Laboratory of Obesity and Metabolic Diseases
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL19415
Series (1)
GSE165350 Exosome derived from liver humanized mice
BioSample SAMN17497284
SRA SRX9921171

Supplementary file Size Download File type/resource
GSM5031952_hm_exosome.counts.tsv.gz 514.2 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap