|
| Status |
Public on Mar 10, 2021 |
| Title |
Humanized Blood Exosomes |
| Sample type |
SRA |
| |
|
| Source name |
liver
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
strain: FRG KO on C57BL/6
host organism: FRG KO mice
xenograft organism: Homo sapiens
tissue: serum
sample type: exosome
|
| Treatment protocol |
HepaCur™serum derived from FRG® KO mice were sent for exosomal RNA-sequencing analysis.
|
| Growth protocol |
FRG® KO on C57BI/6 humanized liver model mice are repopulated with human liver cells from multiple qualified donors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exosome isolation was performed by System Biosciences with the ExoQuick Isolation & RNA Purification Kits
Exosome isolation and subsequent Exo-NGS exosomal RNA-sequencing were performed by System Biosciences.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Sequenced reads were trimmed using trimgalore
The trimmed reads were aligned to a combined human (b38) and mouse (mm10) genome using HISAT2 align with parameters --no-mixed --no-discordant
Aligned reads were transformed into .bam files and sorted by coordinate using sambamba view and sambamba sort.
Read counts were generated using the featureCounts program from the subread package.
Genome_build: GRCh38 and mm10
Supplementary_files_format_and_content: tab-delimited text files include reads counts for the sample
|
| |
|
| Submission date |
Jan 22, 2021 |
| Last update date |
Mar 10, 2021 |
| Contact name |
Haiming Cao |
| E-mail(s) |
haiming.cao@nih.gov
|
| Phone |
3014029032
|
| Organization name |
NIH
|
| Department |
NHLBI
|
| Lab |
Laboratory of Obesity and Metabolic Diseases
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL19415 |
| Series (1) |
| GSE165350 |
Exosome derived from liver humanized mice |
|
| Relations |
| BioSample |
SAMN17497284 |
| SRA |
SRX9921171 |
