|
Status |
Public on Dec 01, 2021 |
Title |
mouse_SCI+saline_1 |
Sample type |
RNA |
|
|
Source name |
muscle
|
Organism |
Mus musculus |
Characteristics |
treatment: SCI+saline time: 2 days post surgery gender: Female tissue: muscle
|
Treatment protocol |
Mice were divided in 4 groups that underwent the following surgeries 1) SCI between vertebrae T12-13+ intramuscular CDTX; 2) SCI+ intramuscular Saline; 3) Sham surgery + intramuscular CDTX; 4) Shamsurgery + Intramuscular saline.
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
Whole hindlinb muscle were sampled 2 days post-surgery and homogenized in 4 ml of Trizol (Invitrogen) with TissueRuptorII (Qiagen Hilden) or IKA T10 basic homogenizer on ice for 30 seconds for 3 times with 10 second intervals followed by incubation at RT for 30 minutes. Tissue debris were removed by centrifuging at 12,000xg and supernatants were mixed with 1/5 volume of chloroform. After spinning down at 12,000xg, 4°C for 15 mins, aqueous phases containing RNA were transferred to fresh tubes for RNA precipitation with isopropanol. The RNA pellets were further washed in 75% ethanol, air-dried and dissolved in RNAse/DNase free water.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
BeadChips and read on a BeadStation 500 System using Beadscan software Version 3.5.31 (Illumina)
|
Description |
9257636011_E
|
Data processing |
Microarray data were analyzed by Illumina Genomestudio software (v2011.1), Gene Expression module (V1.9.0) average normalization algorithms with background subtraction followed by Illumina custom differential analysis algorithms.
|
|
|
Submission date |
Jan 19, 2021 |
Last update date |
Dec 01, 2021 |
Contact name |
Jean-Pierre Levesque |
E-mail(s) |
jp.levesque@mater.uq.edu.au
|
Organization name |
Mater Research Institute-University of Queensland
|
Department |
Mater Research Institute
|
Lab |
Levesque lab
|
Street address |
37 Kent St
|
City |
Brisbane |
State/province |
Queensland |
ZIP/Postal code |
4102 |
Country |
Australia |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE165062 |
Gene expression microarray analysis of whole mouse hamstring muscles to identify genes promoting the devlopment of neurogenic heterotopic ossifications following spinal cord injury |
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