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Sample GSM5024046 Query DataSets for GSM5024046
Status Public on Jan 19, 2021
Title Th1_KO2
Sample type SRA
 
Source name Th1 cells
Organism Mus musculus
Characteristics stain: C57BL/6J
genotype/variation: Mettl3 KO
cell type: splenic Th1 cells
Extracted molecule total RNA
Extraction protocol Tfh cells (SLAM-loCD44+CD62L-CD4+) and Th1 cells (SLAM-hiCD44+CD62L-CD4+) were directly sorted into TRIzol reagent by a FACSAriaII sorter, and total RNA was extracted using standard Qiagen RNease columns.
RNA libraries were prepared for sequencing using standard Illumina protocols。
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing The quality of RNA-Seq libraries was assessed by FastQC (v0.11.5).
RNA-Seq libraries were mapped to mouse genome mm10 (version M17) using TopHat (v2.1.1).
Expression levels of all genes were estimated by HTseq (v0.6.1), and Reads Per Kilobase per Million mapped reads (RPKM) counts were calculated.
Genome_build: mm10
 
Submission date Jan 17, 2021
Last update date Jan 20, 2021
Contact name Shuyang Yu
E-mail(s) ysy@cau.edu.cn
Organization name China Agricultural University
Department College of Biological Sciences
Street address 2 Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100193
Country China
 
Platform ID GPL21273
Series (2)
GSE129648 Regulation of Tfh differentiation by Mettl3 [RNA-seq]
GSE129650 m6A methyltransferase METTL3 regulates Tfh cell differentiation
Relations
BioSample SAMN17367688
SRA SRX9863249

Supplementary file Size Download File type/resource
GSM5024046_Th1_KO_2.geneCounts.txt.gz 214.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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