|
Status |
Public on Dec 01, 2011 |
Title |
quiescent p27delta51 MEFs |
Sample type |
RNA |
|
|
Source name |
delta51
|
Organism |
Mus musculus |
Characteristics |
cell type: MEFs genotype/variation: p27delta51
|
Treatment protocol |
For serum starvation, cells were maintained without serum during 72 h.
|
Growth protocol |
Wt and p27delta51 MEFs were cultured in DMEM supplemented with 10% fetal bovin serum (FBS). All cultures were maintained at 37ºC and 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Kit (QIagen). RNAs from quiescent MEFs from wt or delta51 mice were analyzed using a Bioanalyzer (Agilent). Only samples with RIN number greater than 9 and a ratio A260/280 over than 2 were further used in microarray analysis. Retrotranscription was made followed by labeling with biotin.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45ºC on Affymetrix 430 2.0 murine array. Chips were washed and stained in the Affymetrix Fluidics Station.
|
Scan protocol |
Arrays were scanned using the Scanner Axon 4000B
|
Description |
Gene expression data from p27delta51 MEFs cells
|
Data processing |
The normalization of the arrays was done using the rma method as implemented in the Affy package of R Bioconductor. Quality control measurements were done using ArrayQualityMetrics package of R Bioconductor.
|
|
|
Submission date |
Jan 27, 2010 |
Last update date |
Dec 01, 2011 |
Contact name |
oriol bachs |
E-mail(s) |
obachs@ub.edu, raffaellapippa@ub.edu
|
Phone |
636574156
|
Fax |
0034-934021907
|
Organization name |
University of Barcelona
|
Department |
Cell Biology, Immunology and Neurosciences
|
Street address |
Casanova 143
|
City |
Barcelona |
ZIP/Postal code |
08036 |
Country |
Spain |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE20064 |
Expression data from p27delta51 MEFs cells in quiescence |
GSE20069 |
p27Kip1 regulates transcription of genes involved in tumorigenesis |
|