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Sample GSM501323 Query DataSets for GSM501323
Status Public on Jan 23, 2011
Title HFD_BALB/cByJ_272
Sample type RNA
 
Channel 1
Source name liver sample from BALB/cByJ, HFD
Organism Mus musculus
Characteristics strain: DBA/2J
tissue: liver
diet: high fat diet
Treatment protocol A high-fat diet or EtOH-containing high-fat diet was administered via gastric tube for 28 days.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy5
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
Channel 2
Source name mouse liver RNA (reference)
Organism Mus musculus
Characteristics tissue: liver
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy3
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
 
Hybridization protocol Labeled samples and reference were hybridized to the microarray at 65 degrees for 17 hours and washed with washing solutions.
Scan protocol Arrays were scanned with Agilent Microarray Scanner (Agilent), and the obtained images were analyzed with Agilent Feature Extraction software (v8.5, Agilent Technologies).
Description Batch4
272
Data processing Normalization and filtering were performed with UNC Microarray Database (https://genome.unc.edu/). Inter-batch normalization was carried out using the ComBat procedure.
 
Submission date Jan 26, 2010
Last update date Jan 23, 2011
Contact name Ivan Rusyn
E-mail(s) iir@unc.edu
Phone 919-843-2596
Fax 919-843-2596
URL http://cancer.unc.edu/research/faculty/displayMember.asp?ID=398
Organization name University of North Carolina
Department Gillings School of Global Public Health
Lab Environmental Genomics Lab
Street address 0031 Michael Hooker Research Center
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7431
Country USA
 
Platform ID GPL7202
Series (1)
GSE20052 Gene expression in the liver of ethanol-treated mice via gastric tube in multiple strains

Data table header descriptions
ID_REF
VALUE Lowess-normalized log2 ratio (sample/reference)

Data table
ID_REF VALUE
A_51_P100063 0.324980036
A_51_P100099 -0.074673321
A_51_P100155 -0.064606275
A_51_P100174 -0.168676877
A_51_P100181 0.053815439
A_51_P100227 -0.039901112
A_51_P100246 -0.140992787
A_51_P100289 0.035612707
A_51_P100327 0.011479435
A_51_P100505 -0.307943703
A_51_P100565 0.004793894
A_51_P100573 0.048091148
A_51_P100625 0.118897959
A_51_P100787 -0.041275166
A_51_P100828 -0.069462636
A_51_P100852 -0.179454009
A_51_P100856 0.132878875
A_51_P100866 -0.014350404
A_51_P100991 0.363698047
A_51_P101086 -0.150240087

Total number of rows: 18833

Table truncated, full table size 465 Kbytes.




Supplementary file Size Download File type/resource
GSM501323.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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