|
Status |
Public on Jan 23, 2011 |
Title |
EtOH_NZW/LacJ_217 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
liver sample from NZW/LacJ, EtOH
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/10J tissue: liver diet: ethanol-containing high fat diet
|
Treatment protocol |
A high-fat diet or EtOH-containing high-fat diet was administered via gastric tube for 28 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
|
Label |
Cy5
|
Label protocol |
2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
|
|
|
Channel 2 |
Source name |
mouse liver RNA (reference)
|
Organism |
Mus musculus |
Characteristics |
tissue: liver
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
|
Label |
Cy3
|
Label protocol |
2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
|
|
|
|
Hybridization protocol |
Labeled samples and reference were hybridized to the microarray at 65 degrees for 17 hours and washed with washing solutions.
|
Scan protocol |
Arrays were scanned with Agilent Microarray Scanner (Agilent), and the obtained images were analyzed with Agilent Feature Extraction software (v8.5, Agilent Technologies).
|
Description |
Batch3 217
|
Data processing |
Normalization and filtering were performed with UNC Microarray Database (https://genome.unc.edu/). Inter-batch normalization was carried out using the ComBat procedure.
|
|
|
Submission date |
Jan 26, 2010 |
Last update date |
Jan 23, 2011 |
Contact name |
Ivan Rusyn |
E-mail(s) |
iir@unc.edu
|
Phone |
919-843-2596
|
Fax |
919-843-2596
|
URL |
http://cancer.unc.edu/research/faculty/displayMember.asp?ID=398
|
Organization name |
University of North Carolina
|
Department |
Gillings School of Global Public Health
|
Lab |
Environmental Genomics Lab
|
Street address |
0031 Michael Hooker Research Center
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599-7431 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (1) |
GSE20052 |
Gene expression in the liver of ethanol-treated mice via gastric tube in multiple strains |
|