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Sample GSM501300 Query DataSets for GSM501300
Status Public on Jan 23, 2011
Title EtOH_FVB/NJ_146
Sample type RNA
 
Channel 1
Source name liver sample from FVB/NJ, EtOH
Organism Mus musculus
Characteristics strain: BTBR T+tf/J
tissue: liver
diet: ethanol-containing high fat diet
Treatment protocol A high-fat diet or EtOH-containing high-fat diet was administered via gastric tube for 28 days.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy5
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
Channel 2
Source name mouse liver RNA (reference)
Organism Mus musculus
Characteristics tissue: liver
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy3
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
 
Hybridization protocol Labeled samples and reference were hybridized to the microarray at 65 degrees for 17 hours and washed with washing solutions.
Scan protocol Arrays were scanned with Agilent Microarray Scanner (Agilent), and the obtained images were analyzed with Agilent Feature Extraction software (v8.5, Agilent Technologies).
Description Batch1
146
Data processing Normalization and filtering were performed with UNC Microarray Database (https://genome.unc.edu/). Inter-batch normalization was carried out using the ComBat procedure.
 
Submission date Jan 26, 2010
Last update date Jan 23, 2011
Contact name Ivan Rusyn
E-mail(s) iir@unc.edu
Phone 919-843-2596
Fax 919-843-2596
URL http://cancer.unc.edu/research/faculty/displayMember.asp?ID=398
Organization name University of North Carolina
Department Gillings School of Global Public Health
Lab Environmental Genomics Lab
Street address 0031 Michael Hooker Research Center
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7431
Country USA
 
Platform ID GPL7202
Series (1)
GSE20052 Gene expression in the liver of ethanol-treated mice via gastric tube in multiple strains

Data table header descriptions
ID_REF
VALUE Lowess-normalized log2 ratio (sample/reference)

Data table
ID_REF VALUE
A_51_P100063 -0.114676175
A_51_P100099 -0.248071384
A_51_P100155 -0.27475603
A_51_P100174 -0.210445476
A_51_P100181 0.076787749
A_51_P100227 -0.024886986
A_51_P100246 -0.009521533
A_51_P100289 0.089768721
A_51_P100327 0.33547058
A_51_P100505 0.490340986
A_51_P100565 0.097858161
A_51_P100573 0.138253567
A_51_P100625 0.028888767
A_51_P100787 -0.123667067
A_51_P100828 -0.218284842
A_51_P100852 0.220090758
A_51_P100856 0.341268239
A_51_P100866 -0.071075376
A_51_P100991 0.424760056
A_51_P101086 -0.336824524

Total number of rows: 18833

Table truncated, full table size 466 Kbytes.




Supplementary file Size Download File type/resource
GSM501300.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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