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Sample GSM501294 Query DataSets for GSM501294
Status Public on Jan 23, 2011
Title HFD_DBA/2J_124
Sample type RNA
 
Channel 1
Source name liver sample from DBA/2J, HFD
Organism Mus musculus
Characteristics strain: BALB/cJ
tissue: liver
diet: high fat diet
Treatment protocol A high-fat diet or EtOH-containing high-fat diet was administered via gastric tube for 28 days.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy5
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
Channel 2
Source name mouse liver RNA (reference)
Organism Mus musculus
Characteristics tissue: liver
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy plus mini kit (Qiagen, Valencia, CA).
Label Cy3
Label protocol 2.0 ug of total RNA from each sample, or 2.5 ug from mouse reference RNA (Icoria, Inc., RTP, NC), was labeled with fluorescent dye (Cy5 and Cy3, respectively).
 
 
Hybridization protocol Labeled samples and reference were hybridized to the microarray at 65 degrees for 17 hours and washed with washing solutions.
Scan protocol Arrays were scanned with Agilent Microarray Scanner (Agilent), and the obtained images were analyzed with Agilent Feature Extraction software (v8.5, Agilent Technologies).
Description Batch1
124
Data processing Normalization and filtering were performed with UNC Microarray Database (https://genome.unc.edu/). Inter-batch normalization was carried out using the ComBat procedure.
 
Submission date Jan 26, 2010
Last update date Jan 23, 2011
Contact name Ivan Rusyn
E-mail(s) iir@unc.edu
Phone 919-843-2596
Fax 919-843-2596
URL http://cancer.unc.edu/research/faculty/displayMember.asp?ID=398
Organization name University of North Carolina
Department Gillings School of Global Public Health
Lab Environmental Genomics Lab
Street address 0031 Michael Hooker Research Center
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7431
Country USA
 
Platform ID GPL7202
Series (1)
GSE20052 Gene expression in the liver of ethanol-treated mice via gastric tube in multiple strains

Data table header descriptions
ID_REF
VALUE Lowess-normalized log2 ratio (sample/reference)

Data table
ID_REF VALUE
A_51_P100063 0.533840015
A_51_P100099 -0.10980089
A_51_P100155 -0.100846071
A_51_P100174 0.082539054
A_51_P100181 0.109935687
A_51_P100227 -0.042011059
A_51_P100246 -0.232961702
A_51_P100289 0.08119853
A_51_P100327 0.067250194
A_51_P100505 0.104867405
A_51_P100565 -0.00980979
A_51_P100573 0.029600956
A_51_P100625 0.125904396
A_51_P100787 -0.025383451
A_51_P100828 -0.054151584
A_51_P100852 0.078519299
A_51_P100856 0.205084786
A_51_P100866 -0.025059609
A_51_P100991 0.730574177
A_51_P101086 -0.112934768

Total number of rows: 18833

Table truncated, full table size 466 Kbytes.




Supplementary file Size Download File type/resource
GSM501294.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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