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Sample GSM4990514 Query DataSets for GSM4990514
Status Public on Aug 10, 2021
Title tg(sox10:GFP)
Sample type SRA
 
Source name zebrafish trunk tg(sox10:GFP) positive cells
Organism Danio rerio
Characteristics genotype: tg(sox10:GFP)-ba2
tissue: trunk neural crest cells
developmental stage: 24 hpf
Extracted molecule total RNA
Extraction protocol tg(sox10:GFP) cells were isolated by FACS from dissociated trunks of 20-24hpf zebrafish and submitted to 10x Sequencing
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description trunk tg(sox10:GFP) cells from 20-24hpf zebrafish
Data processing cellranger v3.2
seurat v3.2.2
Genome_build: GRCz11
Supplementary_files_format_and_content: features, barcodes, counts matrix for scRNAseq
Supplementary_files_format_and_content: Cluster assignments
 
Submission date Dec 28, 2020
Last update date Aug 10, 2021
Contact name Ezra Lencer
E-mail(s) lencere@lafayette.edu
Organization name Lafayette College
Department Department of Biology
Lab Lencer Laboratory
Street address 746 High Street
City Easton
State/province PA
ZIP/Postal code 18042
Country USA
 
Platform ID GPL24995
Series (1)
GSE163907 Single cell RNA analysis of trunk neural crest cells in zebrafish
Relations
BioSample SAMN17169818
SRA SRX9739651

Supplementary file Size Download File type/resource
GSM4990514_Clusters.csv.gz 763 b (ftp)(http) CSV
GSM4990514_DifferentialExpression.Wilcox.xls 1.2 Mb (ftp)(http) XLS
GSM4990514_barcodes.tsv.gz 3.2 Kb (ftp)(http) TSV
GSM4990514_features.tsv.gz 237.1 Kb (ftp)(http) TSV
GSM4990514_matrix.mtx.gz 7.4 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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